Identification of osteocyte-selective proteins

Dayong Guo, Andrew Keightley, Jill Guthrie, Patricia A. Veno, Stephen E. Harris, Lynda Bonewald

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Since little is known regarding osteocytes, cells embedded within the mineralized bone matrix, a proteomics approach was used to discover proteins more highly expressed in osteocytes than in osteoblasts to determine osteocyte-specific function. Two proteomic profiles obtained by two different proteomic approaches using total cell lysates from the osteocyte cell line MLO-Y4 and the osteoblast cell line MC3T3 revealed unique differences. Three protein clusters, one related to glycolysis (Phosphoglycerate kinase 1, fructosebisphosphate aldolase A, hypoxia up-regulated 1 [ORP150], triosephosphate isomerase), one to protein folding (Mitochondrial Stress-70 protein, ORP150, Endoplasmin), and one to actin cytoskeleton regulation (Macrophage-capping protein [CapG], destrin, forms of lamin A and vimentin) were identified. Higher protein expression of ORP-150, Cap G, and destrin in MLO-Y4 cells compared with MC3T3 cells was validated by gene expression, Western blotting, and in vivo expression. These proteins were shown to be selective in osteocytes in vivo using immuno-staining of mouse ulnae. Destrin was most highly expressed in embedding osteoid osteocytes, GapG in embedded osteocytes, and ORP150 in deeply embedded osteocytes. In summary, the proteomic approach has yielded important information regarding molecular mechanisms used by osteocytes for embedding in matrix, the formation of dendritic processes, and protection within a hypoxic environment.

Original languageEnglish (US)
Pages (from-to)3688-3698
Number of pages11
JournalProteomics
Volume10
Issue number20
DOIs
StatePublished - Oct 2010
Externally publishedYes

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Osteocytes
Destrin
Proteomics
Proteins
Osteoblasts
Cells
Triose-Phosphate Isomerase
Lamin Type A
Phosphoglycerate Kinase
Protein folding
Fructose-Bisphosphate Aldolase
Macrophages
Vimentin
Gene expression
Actins
Cell Line
Ulna
Bone
Bone Matrix
Mitochondrial Proteins

Keywords

  • Expression
  • Osteoblast
  • Osteocyte
  • Systems biology

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Guo, D., Keightley, A., Guthrie, J., Veno, P. A., Harris, S. E., & Bonewald, L. (2010). Identification of osteocyte-selective proteins. Proteomics, 10(20), 3688-3698. https://doi.org/10.1002/pmic.201000306

Identification of osteocyte-selective proteins. / Guo, Dayong; Keightley, Andrew; Guthrie, Jill; Veno, Patricia A.; Harris, Stephen E.; Bonewald, Lynda.

In: Proteomics, Vol. 10, No. 20, 10.2010, p. 3688-3698.

Research output: Contribution to journalArticle

Guo, D, Keightley, A, Guthrie, J, Veno, PA, Harris, SE & Bonewald, L 2010, 'Identification of osteocyte-selective proteins', Proteomics, vol. 10, no. 20, pp. 3688-3698. https://doi.org/10.1002/pmic.201000306
Guo D, Keightley A, Guthrie J, Veno PA, Harris SE, Bonewald L. Identification of osteocyte-selective proteins. Proteomics. 2010 Oct;10(20):3688-3698. https://doi.org/10.1002/pmic.201000306
Guo, Dayong ; Keightley, Andrew ; Guthrie, Jill ; Veno, Patricia A. ; Harris, Stephen E. ; Bonewald, Lynda. / Identification of osteocyte-selective proteins. In: Proteomics. 2010 ; Vol. 10, No. 20. pp. 3688-3698.
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