Identification of osteopontin in isolated rabbit osteoclasts

Ken ichi Tezuka, Takuya Sato, Hiroshi Kamioka, Peter J. Nijweide, Kayo Tanaka, Tetsu Matsuo, Mitsue Ohta, Noriyoshi Kurihara, Yoshiyuki Hakeda, Masayoshi Kumegawa

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines; however, little is known about protein factors produced by osteoclasts or bone-resorbing cells. A method utilizing the high affinity of osteoclasts for tissue culture dishes was developed to isolate a large number of pure osteoclasts from rabbit long bones. A cDNA library was then constructed from these isolated osteoclasts, and differential cDNA screening was performed between osteoclasts and spleen cells. Two clones representing osteoclast-specific clones, named OC-1 and OC-2, were isolated. By Northern blot analysis, OC-1 was expressed in osteoclasts and in kidneys, whereas OC-2 was specific for osteoclasts. OC-1 was found to encode osteopontin from its nucleotide sequence, and therefore, osteopontin may have other functions for osteoclastic bone resorption besides osteoclast attachment to bone.

Original languageEnglish (US)
Pages (from-to)911-917
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
StatePublished - Jul 31 1992
Externally publishedYes

Fingerprint

Osteopontin
Osteoclasts
Bone
Rabbits
Bone and Bones
Bone Resorption
Clone Cells
Tissue culture
Bone Matrix
Bone Remodeling
Osteoblasts
Gene Library
Osteogenesis
Northern Blotting
Screening
Proteins
Spleen
Nucleotides
Complementary DNA
Cytokines

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Tezuka, K. I., Sato, T., Kamioka, H., Nijweide, P. J., Tanaka, K., Matsuo, T., ... Kumegawa, M. (1992). Identification of osteopontin in isolated rabbit osteoclasts. Biochemical and Biophysical Research Communications, 186(2), 911-917. https://doi.org/10.1016/0006-291X(92)90832-6

Identification of osteopontin in isolated rabbit osteoclasts. / Tezuka, Ken ichi; Sato, Takuya; Kamioka, Hiroshi; Nijweide, Peter J.; Tanaka, Kayo; Matsuo, Tetsu; Ohta, Mitsue; Kurihara, Noriyoshi; Hakeda, Yoshiyuki; Kumegawa, Masayoshi.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 2, 31.07.1992, p. 911-917.

Research output: Contribution to journalArticle

Tezuka, KI, Sato, T, Kamioka, H, Nijweide, PJ, Tanaka, K, Matsuo, T, Ohta, M, Kurihara, N, Hakeda, Y & Kumegawa, M 1992, 'Identification of osteopontin in isolated rabbit osteoclasts', Biochemical and Biophysical Research Communications, vol. 186, no. 2, pp. 911-917. https://doi.org/10.1016/0006-291X(92)90832-6
Tezuka, Ken ichi ; Sato, Takuya ; Kamioka, Hiroshi ; Nijweide, Peter J. ; Tanaka, Kayo ; Matsuo, Tetsu ; Ohta, Mitsue ; Kurihara, Noriyoshi ; Hakeda, Yoshiyuki ; Kumegawa, Masayoshi. / Identification of osteopontin in isolated rabbit osteoclasts. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 2. pp. 911-917.
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