Identification of porcine Lhx3 and SF1 as candidate genes for QTL affecting growth and reproduction traits in swine

T. P L Smith, A. D. Showalter, K. W. Sloop, G. A. Rohrer, S. C. Fahrenkrug, B. C. Meier, Simon Rhodes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The distal portion of the long arm of porcine chromosome 1 has been shown to harbour several quantitative trait loci affecting growth and reproductive traits in swine. In order to identify potential candidate genes that might underlie these effects, a comparative mapping analysis was undertaken to define the extent of orthologous segments of human chromosome 9. A microsatellite associated with heat shock protein (HSP) A5 was used to define the proximal boundary of the quantitative trait loci (QTL) region, which suggests the human orthologue of the gene(s) responsible for the observed effects lies between HSPA5 and the q arm telomere of human chromosome 9. Examination of this region revealed two candidate genes with known roles in production of hormones essential to growth and reproductive function. The steroidogenic factor 1 and Lhx3 LIM homeodomain transcription factor genes were mapped to 123 and 155 cM, respectively, of the Sus scrofa chromosome 1 (SSC1) linkage group, placing both genes within the confidence interval for the observed QTL. To further evaluate Lhx3, we examined the expression profile during porcine embryonic development. Low levels were detected at early embryonic stages, when development of the nervous system is proceeding. A transient increase in expression level is observed during the time of pituitary organogenesis and again at the time of differentiation of anterior pituitary cells, with relatively high levels of expression persisting in the adult pituitary gland. This ontology is consistent with Lhx3 being a candidate gene for the QTL.

Original languageEnglish
Pages (from-to)344-350
Number of pages7
JournalAnimal Genetics
Volume32
Issue number6
DOIs
StatePublished - 2001

Fingerprint

Quantitative Trait Loci
Reproduction
quantitative trait loci
Swine
swine
Growth
Chromosomes, Human, Pair 9
Genes
Chromosomes, Human, Pair 1
chromosomes
Human Chromosomes
genes
Steroidogenic Factor 1
Sus scrofa
Organogenesis
Telomere
Pituitary Gland
Heat-Shock Proteins
anterior pituitary
neurodevelopment

Keywords

  • Growth
  • Mapping
  • Pituitary
  • QTL
  • Reproduction

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Identification of porcine Lhx3 and SF1 as candidate genes for QTL affecting growth and reproduction traits in swine. / Smith, T. P L; Showalter, A. D.; Sloop, K. W.; Rohrer, G. A.; Fahrenkrug, S. C.; Meier, B. C.; Rhodes, Simon.

In: Animal Genetics, Vol. 32, No. 6, 2001, p. 344-350.

Research output: Contribution to journalArticle

Smith, T. P L ; Showalter, A. D. ; Sloop, K. W. ; Rohrer, G. A. ; Fahrenkrug, S. C. ; Meier, B. C. ; Rhodes, Simon. / Identification of porcine Lhx3 and SF1 as candidate genes for QTL affecting growth and reproduction traits in swine. In: Animal Genetics. 2001 ; Vol. 32, No. 6. pp. 344-350.
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