Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening

Karuna C. Nallani, William Sullivan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Toxoplasma gondii is an opportunistic protozoan parasite that differentiates into latent cysts (bradyzoite) that can be reactivated during immunosuppression. TgSRCAP (Toxoplasma gondii Snf2-related CBP activator protein) is a SWI2/SNF2 family chromatin remodeler whose expression increases during cyst development. Identifying the proteins associating with TgSRCAP during the pre-cyst stage (tachyzoite) will increase our understanding of how parasite differentiation is initiated. We employed the yeast two-hybrid system to identify proteins that may interact directly with TgSRCAP. A stretch of 1,060 amino acids between ATPase subdomains IV and V of TgSRCAP was chosen as "bait" since the corresponding region in human SRCAP interacts with other proteins, including CREB binding protein. We have identified several novel parasite-specific transcription factors predicted to be in the T. gondii genome. Metabolic enzymes that may participate in cyst development were also identified as interacting with TgSRCAP.

Original languageEnglish
Pages (from-to)236-242
Number of pages7
JournalParasitology Research
Volume95
Issue number4
DOIs
StatePublished - Mar 2005

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two hybrid system techniques
Toxoplasma
Toxoplasma gondii
Yeasts
yeasts
Cysts
Proteins
proteins
Parasites
parasites
CREB-Binding Protein
tachyzoites
Two-Hybrid System Techniques
immunosuppression
baits
Immunosuppression
Chromatin
adenosinetriphosphatase
Protozoa
chromatin

ASJC Scopus subject areas

  • Parasitology

Cite this

Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening. / Nallani, Karuna C.; Sullivan, William.

In: Parasitology Research, Vol. 95, No. 4, 03.2005, p. 236-242.

Research output: Contribution to journalArticle

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