Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism

A nontargeted proteomic study

María Insenser, Rafael Montes-Nieto, M. Ángeles Martínez-García, Elena Fernandez Durań, Carmen Santiuste, Vicente Goḿez, Jeffrey Kline, Héctor F. Escobar-Morreale, David Jimeńez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Risk stratification of patients with pulmonary embolism (PE) may identify patients at high risk of early death who may benefit from more intensive surveillance or aggressive therapy. Nontargeted proteomics may identify biomarkers useful for the risk stratification of patients with acute symptomatic pulmonary embolism (PE). We studied 6 patients presenting with low-risk PE and 6 patients presenting with intermediate (n = 3) or high-risk (n = 3) PE. Two-dimensional difference gel electrophoresis was used to compare their plasma protein abundances. Candidate protein markers were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry. A panel of four biomarkers (haptoglobin, hemopexin, α2-macroglobulin, and Ig α1-chain C region) showed differences in plasma abundance among patients with acute symptomatic PE of different severity. Haptoglobin and hemopexin were decreased, whereas α2-macroglobulin and Ig α1-chain C region were increased, in patients with high or intermediate-risk PE compared with low-risk PE patient. In a separate clinical population consisting of 104 adults with acute PE, serum haptoglobin concentrations had an 85% chance of correctly identifying patients with high-risk PE according to receiving operating characteristics curve analysis. Moreover, serum haptoglobin concentrations ≤1 g/l showed an 80% sensitivity and a 96% specificity for the diagnosis of high-risk PE. Nontargeted proteomics identified protein biomarkers for the severity of PE that are involved in iron metabolism pathways and acute-phase response. Among them, reduced serum haptoglobin concentrations show a high accuracy for the biochemical detection of high-risk PE.

Original languageEnglish
Article numbere100902
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 30 2014

Fingerprint

Haptoglobins
haptoglobins
embolism
Biomarkers
Pulmonary Embolism
Proteomics
proteomics
biomarkers
lungs
Hemopexin
Macroglobulins
macroglobulins
Serum
Two-Dimensional Difference Gel Electrophoresis
Acute-Phase Reaction
Electrophoresis
Metabolism
matrix-assisted laser desorption-ionization mass spectrometry
Ionization
Mass spectrometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Insenser, M., Montes-Nieto, R., Martínez-García, M. Á., Durań, E. F., Santiuste, C., Goḿez, V., ... Jimeńez, D. (2014). Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: A nontargeted proteomic study. PLoS One, 9(6), [e100902]. https://doi.org/10.1371/journal.pone.0100902

Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism : A nontargeted proteomic study. / Insenser, María; Montes-Nieto, Rafael; Martínez-García, M. Ángeles; Durań, Elena Fernandez; Santiuste, Carmen; Goḿez, Vicente; Kline, Jeffrey; Escobar-Morreale, Héctor F.; Jimeńez, David.

In: PLoS One, Vol. 9, No. 6, e100902, 30.06.2014.

Research output: Contribution to journalArticle

Insenser, M, Montes-Nieto, R, Martínez-García, MÁ, Durań, EF, Santiuste, C, Goḿez, V, Kline, J, Escobar-Morreale, HF & Jimeńez, D 2014, 'Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: A nontargeted proteomic study', PLoS One, vol. 9, no. 6, e100902. https://doi.org/10.1371/journal.pone.0100902
Insenser, María ; Montes-Nieto, Rafael ; Martínez-García, M. Ángeles ; Durań, Elena Fernandez ; Santiuste, Carmen ; Goḿez, Vicente ; Kline, Jeffrey ; Escobar-Morreale, Héctor F. ; Jimeńez, David. / Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism : A nontargeted proteomic study. In: PLoS One. 2014 ; Vol. 9, No. 6.
@article{59fa99165662499eb47a87db11f770cf,
title = "Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: A nontargeted proteomic study",
abstract = "Risk stratification of patients with pulmonary embolism (PE) may identify patients at high risk of early death who may benefit from more intensive surveillance or aggressive therapy. Nontargeted proteomics may identify biomarkers useful for the risk stratification of patients with acute symptomatic pulmonary embolism (PE). We studied 6 patients presenting with low-risk PE and 6 patients presenting with intermediate (n = 3) or high-risk (n = 3) PE. Two-dimensional difference gel electrophoresis was used to compare their plasma protein abundances. Candidate protein markers were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry. A panel of four biomarkers (haptoglobin, hemopexin, α2-macroglobulin, and Ig α1-chain C region) showed differences in plasma abundance among patients with acute symptomatic PE of different severity. Haptoglobin and hemopexin were decreased, whereas α2-macroglobulin and Ig α1-chain C region were increased, in patients with high or intermediate-risk PE compared with low-risk PE patient. In a separate clinical population consisting of 104 adults with acute PE, serum haptoglobin concentrations had an 85{\%} chance of correctly identifying patients with high-risk PE according to receiving operating characteristics curve analysis. Moreover, serum haptoglobin concentrations ≤1 g/l showed an 80{\%} sensitivity and a 96{\%} specificity for the diagnosis of high-risk PE. Nontargeted proteomics identified protein biomarkers for the severity of PE that are involved in iron metabolism pathways and acute-phase response. Among them, reduced serum haptoglobin concentrations show a high accuracy for the biochemical detection of high-risk PE.",
author = "Mar{\'i}a Insenser and Rafael Montes-Nieto and Mart{\'i}nez-Garc{\'i}a, {M. {\'A}ngeles} and Durań, {Elena Fernandez} and Carmen Santiuste and Vicente Goḿez and Jeffrey Kline and Escobar-Morreale, {H{\'e}ctor F.} and David Jimeńez",
year = "2014",
month = "6",
day = "30",
doi = "10.1371/journal.pone.0100902",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism

T2 - A nontargeted proteomic study

AU - Insenser, María

AU - Montes-Nieto, Rafael

AU - Martínez-García, M. Ángeles

AU - Durań, Elena Fernandez

AU - Santiuste, Carmen

AU - Goḿez, Vicente

AU - Kline, Jeffrey

AU - Escobar-Morreale, Héctor F.

AU - Jimeńez, David

PY - 2014/6/30

Y1 - 2014/6/30

N2 - Risk stratification of patients with pulmonary embolism (PE) may identify patients at high risk of early death who may benefit from more intensive surveillance or aggressive therapy. Nontargeted proteomics may identify biomarkers useful for the risk stratification of patients with acute symptomatic pulmonary embolism (PE). We studied 6 patients presenting with low-risk PE and 6 patients presenting with intermediate (n = 3) or high-risk (n = 3) PE. Two-dimensional difference gel electrophoresis was used to compare their plasma protein abundances. Candidate protein markers were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry. A panel of four biomarkers (haptoglobin, hemopexin, α2-macroglobulin, and Ig α1-chain C region) showed differences in plasma abundance among patients with acute symptomatic PE of different severity. Haptoglobin and hemopexin were decreased, whereas α2-macroglobulin and Ig α1-chain C region were increased, in patients with high or intermediate-risk PE compared with low-risk PE patient. In a separate clinical population consisting of 104 adults with acute PE, serum haptoglobin concentrations had an 85% chance of correctly identifying patients with high-risk PE according to receiving operating characteristics curve analysis. Moreover, serum haptoglobin concentrations ≤1 g/l showed an 80% sensitivity and a 96% specificity for the diagnosis of high-risk PE. Nontargeted proteomics identified protein biomarkers for the severity of PE that are involved in iron metabolism pathways and acute-phase response. Among them, reduced serum haptoglobin concentrations show a high accuracy for the biochemical detection of high-risk PE.

AB - Risk stratification of patients with pulmonary embolism (PE) may identify patients at high risk of early death who may benefit from more intensive surveillance or aggressive therapy. Nontargeted proteomics may identify biomarkers useful for the risk stratification of patients with acute symptomatic pulmonary embolism (PE). We studied 6 patients presenting with low-risk PE and 6 patients presenting with intermediate (n = 3) or high-risk (n = 3) PE. Two-dimensional difference gel electrophoresis was used to compare their plasma protein abundances. Candidate protein markers were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry. A panel of four biomarkers (haptoglobin, hemopexin, α2-macroglobulin, and Ig α1-chain C region) showed differences in plasma abundance among patients with acute symptomatic PE of different severity. Haptoglobin and hemopexin were decreased, whereas α2-macroglobulin and Ig α1-chain C region were increased, in patients with high or intermediate-risk PE compared with low-risk PE patient. In a separate clinical population consisting of 104 adults with acute PE, serum haptoglobin concentrations had an 85% chance of correctly identifying patients with high-risk PE according to receiving operating characteristics curve analysis. Moreover, serum haptoglobin concentrations ≤1 g/l showed an 80% sensitivity and a 96% specificity for the diagnosis of high-risk PE. Nontargeted proteomics identified protein biomarkers for the severity of PE that are involved in iron metabolism pathways and acute-phase response. Among them, reduced serum haptoglobin concentrations show a high accuracy for the biochemical detection of high-risk PE.

UR - http://www.scopus.com/inward/record.url?scp=84903650115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903650115&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0100902

DO - 10.1371/journal.pone.0100902

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e100902

ER -