Identification of regenerative tissue cables using in vivo MRI after spinal cord hemisection and Schwann cell bridging transplantation

Christopher Iannotti, Huayin Li, Mark Stemmler, William H. Perman, Xiao-Ming Xu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The purpose of this study was to examine the feasibility of a non-invasive in vivo magnetic resonance imaging (MRI) procedure, performed at 1.5 T, to detect regenerative tissue cables in a rat spinal cord hemisection and Schwann cell (SC) bridging transplantation paradigm. Two months after implantation of a SC-seeded guidance channel (1.25 mm in diameter and 3.0 mm in length) into a T8 spinal cord hemisection-gap lesion, axial fast-spin echo (FSE) T2-weighted MR imaging (T2WI) was performed. Axial T2WI through the graft identified a circular area of low intensity surrounded by high-intensity signal within the guidance channel lumen. Correlative histological assessments of Toluidine blue-stained sections confirmed that the low-intensity signal represented a tissue cable, which, in most cases, contained a substantial number of myelinated axons oriented along the rostro-caudal axis of the spinal cord. The percentage of guidance channel cross-sectional area occupied by the tissue cable, expressed as the tissue cable index (TCI), was also determined from histological sections. Linear regression analysis of the TCI plotted relative to the number of myelinated axons revealed a strong positive correlation (r2 = 0.85) between these two outcome measures. In addition, the sensitivity of MRI to detect regenerative tissue cables within guidance channels was 86%. These results demonstrate that (1) 1.5 T MR imaging performed 2 months after spinal cord hemisection and SC bridging transplantation is sensitive in detecting low-intensity regenerative tissue cables, and (2) the TCI strongly correlates with the extent of axonal regeneration into implanted SCseeded guidance channels.

Original languageEnglish (US)
Pages (from-to)1543-1554
Number of pages12
JournalJournal of Neurotrauma
Volume19
Issue number12
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Schwann Cells
Cell Transplantation
Spinal Cord
Magnetic Resonance Imaging
Axons
Tolonium Chloride
Regeneration
Linear Models
Regression Analysis
Outcome Assessment (Health Care)
Transplants

Keywords

  • Axonal regeneration
  • Cerebrospinal fluid
  • Guidance channel
  • Magnetic resonance imaging
  • Schwann cell
  • Spinal cord injury
  • Transplantation

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Identification of regenerative tissue cables using in vivo MRI after spinal cord hemisection and Schwann cell bridging transplantation. / Iannotti, Christopher; Li, Huayin; Stemmler, Mark; Perman, William H.; Xu, Xiao-Ming.

In: Journal of Neurotrauma, Vol. 19, No. 12, 01.12.2002, p. 1543-1554.

Research output: Contribution to journalArticle

Iannotti, Christopher ; Li, Huayin ; Stemmler, Mark ; Perman, William H. ; Xu, Xiao-Ming. / Identification of regenerative tissue cables using in vivo MRI after spinal cord hemisection and Schwann cell bridging transplantation. In: Journal of Neurotrauma. 2002 ; Vol. 19, No. 12. pp. 1543-1554.
@article{decf1f0245b445ddb4bb9b3e2cc78bfd,
title = "Identification of regenerative tissue cables using in vivo MRI after spinal cord hemisection and Schwann cell bridging transplantation",
abstract = "The purpose of this study was to examine the feasibility of a non-invasive in vivo magnetic resonance imaging (MRI) procedure, performed at 1.5 T, to detect regenerative tissue cables in a rat spinal cord hemisection and Schwann cell (SC) bridging transplantation paradigm. Two months after implantation of a SC-seeded guidance channel (1.25 mm in diameter and 3.0 mm in length) into a T8 spinal cord hemisection-gap lesion, axial fast-spin echo (FSE) T2-weighted MR imaging (T2WI) was performed. Axial T2WI through the graft identified a circular area of low intensity surrounded by high-intensity signal within the guidance channel lumen. Correlative histological assessments of Toluidine blue-stained sections confirmed that the low-intensity signal represented a tissue cable, which, in most cases, contained a substantial number of myelinated axons oriented along the rostro-caudal axis of the spinal cord. The percentage of guidance channel cross-sectional area occupied by the tissue cable, expressed as the tissue cable index (TCI), was also determined from histological sections. Linear regression analysis of the TCI plotted relative to the number of myelinated axons revealed a strong positive correlation (r2 = 0.85) between these two outcome measures. In addition, the sensitivity of MRI to detect regenerative tissue cables within guidance channels was 86{\%}. These results demonstrate that (1) 1.5 T MR imaging performed 2 months after spinal cord hemisection and SC bridging transplantation is sensitive in detecting low-intensity regenerative tissue cables, and (2) the TCI strongly correlates with the extent of axonal regeneration into implanted SCseeded guidance channels.",
keywords = "Axonal regeneration, Cerebrospinal fluid, Guidance channel, Magnetic resonance imaging, Schwann cell, Spinal cord injury, Transplantation",
author = "Christopher Iannotti and Huayin Li and Mark Stemmler and Perman, {William H.} and Xiao-Ming Xu",
year = "2002",
month = "12",
day = "1",
language = "English (US)",
volume = "19",
pages = "1543--1554",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "12",

}

TY - JOUR

T1 - Identification of regenerative tissue cables using in vivo MRI after spinal cord hemisection and Schwann cell bridging transplantation

AU - Iannotti, Christopher

AU - Li, Huayin

AU - Stemmler, Mark

AU - Perman, William H.

AU - Xu, Xiao-Ming

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The purpose of this study was to examine the feasibility of a non-invasive in vivo magnetic resonance imaging (MRI) procedure, performed at 1.5 T, to detect regenerative tissue cables in a rat spinal cord hemisection and Schwann cell (SC) bridging transplantation paradigm. Two months after implantation of a SC-seeded guidance channel (1.25 mm in diameter and 3.0 mm in length) into a T8 spinal cord hemisection-gap lesion, axial fast-spin echo (FSE) T2-weighted MR imaging (T2WI) was performed. Axial T2WI through the graft identified a circular area of low intensity surrounded by high-intensity signal within the guidance channel lumen. Correlative histological assessments of Toluidine blue-stained sections confirmed that the low-intensity signal represented a tissue cable, which, in most cases, contained a substantial number of myelinated axons oriented along the rostro-caudal axis of the spinal cord. The percentage of guidance channel cross-sectional area occupied by the tissue cable, expressed as the tissue cable index (TCI), was also determined from histological sections. Linear regression analysis of the TCI plotted relative to the number of myelinated axons revealed a strong positive correlation (r2 = 0.85) between these two outcome measures. In addition, the sensitivity of MRI to detect regenerative tissue cables within guidance channels was 86%. These results demonstrate that (1) 1.5 T MR imaging performed 2 months after spinal cord hemisection and SC bridging transplantation is sensitive in detecting low-intensity regenerative tissue cables, and (2) the TCI strongly correlates with the extent of axonal regeneration into implanted SCseeded guidance channels.

AB - The purpose of this study was to examine the feasibility of a non-invasive in vivo magnetic resonance imaging (MRI) procedure, performed at 1.5 T, to detect regenerative tissue cables in a rat spinal cord hemisection and Schwann cell (SC) bridging transplantation paradigm. Two months after implantation of a SC-seeded guidance channel (1.25 mm in diameter and 3.0 mm in length) into a T8 spinal cord hemisection-gap lesion, axial fast-spin echo (FSE) T2-weighted MR imaging (T2WI) was performed. Axial T2WI through the graft identified a circular area of low intensity surrounded by high-intensity signal within the guidance channel lumen. Correlative histological assessments of Toluidine blue-stained sections confirmed that the low-intensity signal represented a tissue cable, which, in most cases, contained a substantial number of myelinated axons oriented along the rostro-caudal axis of the spinal cord. The percentage of guidance channel cross-sectional area occupied by the tissue cable, expressed as the tissue cable index (TCI), was also determined from histological sections. Linear regression analysis of the TCI plotted relative to the number of myelinated axons revealed a strong positive correlation (r2 = 0.85) between these two outcome measures. In addition, the sensitivity of MRI to detect regenerative tissue cables within guidance channels was 86%. These results demonstrate that (1) 1.5 T MR imaging performed 2 months after spinal cord hemisection and SC bridging transplantation is sensitive in detecting low-intensity regenerative tissue cables, and (2) the TCI strongly correlates with the extent of axonal regeneration into implanted SCseeded guidance channels.

KW - Axonal regeneration

KW - Cerebrospinal fluid

KW - Guidance channel

KW - Magnetic resonance imaging

KW - Schwann cell

KW - Spinal cord injury

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=0036922635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036922635&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 1543

EP - 1554

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 12

ER -