Identification of signature genes for detecting hedgehog pathway activation in esophageal cancer

Ling Yang, Yuehong Bian, Shuhong Huang, Xiaoli Ma, Chi Zhang, Xiulan Su, Zi Jiang Chen, Jingwu Xie, Hongwei Zhang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The hedgehog signaling pathway plays an important role in cell growth and differentiation both in normal embryonic development and in tumors. Our previous work shows that hedgehog pathway is frequently activated in esophageal cancers. To further elucidate the role of hedgehog pathway in esophageal cancers we examined the expression of the target genes, hedgehog-interacting protein (HIP) and platelet derived growth factor receptor alpha (PDGFRα) and hedgehog signaling molecules, smoothened (SMO), suppressor of fused (Su(Fu)) in the specimens using in-situ hybridization and RT-PCR. We found that HIP, PDGFRα, SMO and Su(Fu) gene highly expressed in the primary esophageal squamous cell carcinomas but not in normal esophageal tissue. The transcripts of HIP, PDGFRα and SMO were expressed in 13 of 15 esophageal cancers. Su(Fu) expression was missing in 2 esophageal cancers. The results from in-situ hybridization were further confirmed by RT-PCR. Our results revealed a set of genes for detecting hedgehog signaling activation in esophageal cancer.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalPathology & Oncology Research
Volume17
Issue number2
DOIs
StatePublished - Jun 2011

Fingerprint

Esophageal Neoplasms
Hedgehog Proteins
Platelet-Derived Growth Factor alpha Receptor
Genes
In Situ Hybridization
Polymerase Chain Reaction
Embryonic Development
Cell Differentiation
Gene Expression
Growth
Neoplasms

Keywords

  • Esophageal cancer
  • Hedgehog
  • Hedgehog-interacting protein
  • Platelet derived growth factor receptor alpha
  • Smoothened
  • Suppressor of fused

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Identification of signature genes for detecting hedgehog pathway activation in esophageal cancer. / Yang, Ling; Bian, Yuehong; Huang, Shuhong; Ma, Xiaoli; Zhang, Chi; Su, Xiulan; Chen, Zi Jiang; Xie, Jingwu; Zhang, Hongwei.

In: Pathology & Oncology Research, Vol. 17, No. 2, 06.2011, p. 387-391.

Research output: Contribution to journalArticle

Yang, Ling ; Bian, Yuehong ; Huang, Shuhong ; Ma, Xiaoli ; Zhang, Chi ; Su, Xiulan ; Chen, Zi Jiang ; Xie, Jingwu ; Zhang, Hongwei. / Identification of signature genes for detecting hedgehog pathway activation in esophageal cancer. In: Pathology & Oncology Research. 2011 ; Vol. 17, No. 2. pp. 387-391.
@article{55f90fec31d8410ba209b3266e29238d,
title = "Identification of signature genes for detecting hedgehog pathway activation in esophageal cancer",
abstract = "The hedgehog signaling pathway plays an important role in cell growth and differentiation both in normal embryonic development and in tumors. Our previous work shows that hedgehog pathway is frequently activated in esophageal cancers. To further elucidate the role of hedgehog pathway in esophageal cancers we examined the expression of the target genes, hedgehog-interacting protein (HIP) and platelet derived growth factor receptor alpha (PDGFRα) and hedgehog signaling molecules, smoothened (SMO), suppressor of fused (Su(Fu)) in the specimens using in-situ hybridization and RT-PCR. We found that HIP, PDGFRα, SMO and Su(Fu) gene highly expressed in the primary esophageal squamous cell carcinomas but not in normal esophageal tissue. The transcripts of HIP, PDGFRα and SMO were expressed in 13 of 15 esophageal cancers. Su(Fu) expression was missing in 2 esophageal cancers. The results from in-situ hybridization were further confirmed by RT-PCR. Our results revealed a set of genes for detecting hedgehog signaling activation in esophageal cancer.",
keywords = "Esophageal cancer, Hedgehog, Hedgehog-interacting protein, Platelet derived growth factor receptor alpha, Smoothened, Suppressor of fused",
author = "Ling Yang and Yuehong Bian and Shuhong Huang and Xiaoli Ma and Chi Zhang and Xiulan Su and Chen, {Zi Jiang} and Jingwu Xie and Hongwei Zhang",
year = "2011",
month = "6",
doi = "10.1007/s12253-010-9337-8",
language = "English",
volume = "17",
pages = "387--391",
journal = "Pathology and Oncology Research",
issn = "1219-4956",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Identification of signature genes for detecting hedgehog pathway activation in esophageal cancer

AU - Yang, Ling

AU - Bian, Yuehong

AU - Huang, Shuhong

AU - Ma, Xiaoli

AU - Zhang, Chi

AU - Su, Xiulan

AU - Chen, Zi Jiang

AU - Xie, Jingwu

AU - Zhang, Hongwei

PY - 2011/6

Y1 - 2011/6

N2 - The hedgehog signaling pathway plays an important role in cell growth and differentiation both in normal embryonic development and in tumors. Our previous work shows that hedgehog pathway is frequently activated in esophageal cancers. To further elucidate the role of hedgehog pathway in esophageal cancers we examined the expression of the target genes, hedgehog-interacting protein (HIP) and platelet derived growth factor receptor alpha (PDGFRα) and hedgehog signaling molecules, smoothened (SMO), suppressor of fused (Su(Fu)) in the specimens using in-situ hybridization and RT-PCR. We found that HIP, PDGFRα, SMO and Su(Fu) gene highly expressed in the primary esophageal squamous cell carcinomas but not in normal esophageal tissue. The transcripts of HIP, PDGFRα and SMO were expressed in 13 of 15 esophageal cancers. Su(Fu) expression was missing in 2 esophageal cancers. The results from in-situ hybridization were further confirmed by RT-PCR. Our results revealed a set of genes for detecting hedgehog signaling activation in esophageal cancer.

AB - The hedgehog signaling pathway plays an important role in cell growth and differentiation both in normal embryonic development and in tumors. Our previous work shows that hedgehog pathway is frequently activated in esophageal cancers. To further elucidate the role of hedgehog pathway in esophageal cancers we examined the expression of the target genes, hedgehog-interacting protein (HIP) and platelet derived growth factor receptor alpha (PDGFRα) and hedgehog signaling molecules, smoothened (SMO), suppressor of fused (Su(Fu)) in the specimens using in-situ hybridization and RT-PCR. We found that HIP, PDGFRα, SMO and Su(Fu) gene highly expressed in the primary esophageal squamous cell carcinomas but not in normal esophageal tissue. The transcripts of HIP, PDGFRα and SMO were expressed in 13 of 15 esophageal cancers. Su(Fu) expression was missing in 2 esophageal cancers. The results from in-situ hybridization were further confirmed by RT-PCR. Our results revealed a set of genes for detecting hedgehog signaling activation in esophageal cancer.

KW - Esophageal cancer

KW - Hedgehog

KW - Hedgehog-interacting protein

KW - Platelet derived growth factor receptor alpha

KW - Smoothened

KW - Suppressor of fused

UR - http://www.scopus.com/inward/record.url?scp=80051671696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051671696&partnerID=8YFLogxK

U2 - 10.1007/s12253-010-9337-8

DO - 10.1007/s12253-010-9337-8

M3 - Article

C2 - 21210262

AN - SCOPUS:80051671696

VL - 17

SP - 387

EP - 391

JO - Pathology and Oncology Research

JF - Pathology and Oncology Research

SN - 1219-4956

IS - 2

ER -