Identification of signature genes for detecting hedgehog signaling activation in gastric cancer

Ling Yang, Shuhong Huang, Yuehong Bian, Xiaoli Ma, Hongwei Zhang, Jingwu Xie

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The aim of this study was to investigate the expression of hedgehog signaling molecules in gastric cancer. In situ hybridization, immunohistochemistry and RT-PCRfor hedgehog signaling molecules, smoothened (SMO), suppressor of fused [Su(Fu)], and the target genes hedgehog-interacting protein (HIP) and platelet-derived growth factor receptor α (PDGFRα) were performed in 30 gastric cancer and two gastritis specimens. Using in situ hybridization, SMOexpression was detected in 18/30 cancerous specimens (60%) as well as in 1/2 gastritis specimens (50%). Su(Fu) was expressed in 15/30 (50%), HIP in 14/30 (∼47%), and PDGFRα in 6/30 (20%) gastric cancer specimens. Despite the heterogeneous expression pattern, SMO, Su(Fu) and PDGFRá transcripts were highly correlated with the HIP transcript in the 30 gastric cancer specimens (p=0.0006, 0.0003 and 0.0441, respectively). Results from the in situ hybridization were further confirmed by RT-PCR for the expression of all of the genes and by immunohistochemistry for SMOexpression. The findings revealed a set of genes for detecting Hh signaling activation in gastric cancer.

Original languageEnglish (US)
Pages (from-to)473-478
Number of pages6
JournalMolecular Medicine Reports
Volume3
Issue number3
DOIs
StatePublished - May 1 2010

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Keywords

  • Gastric cancer
  • Hedgehog
  • Hedgehog-interacting protein
  • PDGFRá
  • Smoothened
  • Suppressor of fused

ASJC Scopus subject areas

  • Biochemistry
  • Cancer Research
  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Oncology

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