Identification of sn-2 acetyl glycerophosphocholines in human keratinocytes

Jeffrey B. Travers, Christopher Johnson, Keith L. Clay, Kathleen Harrison, Tami Zekman, Joseph G. Morelli, Robert C. Murphy

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Evidence is accumulating suggesting that platelet-activating factor plays a role in inflammatory dermatoses. Mass spectrometric methods were used to examine the molecular species of sn-2 acetyl glycerophosphocholines (GPC) synthesized by primary cultures of human neonatal foreskin-derived keratinocytes. Ionophore-stimulated keratinocytes synthesize both 1-alkyl and 1-acyl sn-2 acetyl-GPC, and the relative amounts were as follows: hexadecyl > palmitoyl > octadecyl > stearoyl at the sn-1 position. PAF synthesis in the keratinocytederived cell line HaCaT was inhibited by dexamethasone, suggesting that the anti-inflammatory effects of glucocorticosteroids in inflammatory dermatoses might be in part related to the inhibition of the synthesis of mediators such as PAF.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalJournal of Lipid Mediators and Cell Signalling
Volume16
Issue number3
DOIs
StatePublished - Jul 1 1997

Keywords

  • Acyl-PAF
  • Human glucocccorticosteroids
  • Keratinocytes
  • Mass spectometry
  • Platelet-activating factor

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

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