Identification of sn-2 acetyl glycerophosphocholines in human keratinocytes

Jeffrey B. Travers, Christopher Johnson, Keith L. Clay, Kathleen Harrison, Tami Zekman, Joseph G. Morelli, Robert C. Murphy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Evidence is accumulating suggesting that platelet-activating factor plays a role in inflammatory dermatoses. Mass spectrometric methods were used to examine the molecular species of sn-2 acetyl glycerophosphocholines (GPC) synthesized by primary cultures of human neonatal foreskin-derived keratinocytes. Ionophore-stimulated keratinocytes synthesize both 1-alkyl and 1-acyl sn-2 acetyl-GPC, and the relative amounts were as follows: hexadecyl > palmitoyl > octadecyl > stearoyl at the sn-1 position. PAF synthesis in the keratinocytederived cell line HaCaT was inhibited by dexamethasone, suggesting that the anti-inflammatory effects of glucocorticosteroids in inflammatory dermatoses might be in part related to the inhibition of the synthesis of mediators such as PAF.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalJournal of Lipid Mediators and Cell Signalling
Volume16
Issue number3
DOIs
StatePublished - Jul 1 1997

Fingerprint

Platelet Activating Factor
Keratinocytes
Skin Diseases
Foreskin
Ionophores
Dexamethasone
Anti-Inflammatory Agents
Cells
Cell Line

Keywords

  • Acyl-PAF
  • Human glucocccorticosteroids
  • Keratinocytes
  • Mass spectometry
  • Platelet-activating factor

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

Identification of sn-2 acetyl glycerophosphocholines in human keratinocytes. / Travers, Jeffrey B.; Johnson, Christopher; Clay, Keith L.; Harrison, Kathleen; Zekman, Tami; Morelli, Joseph G.; Murphy, Robert C.

In: Journal of Lipid Mediators and Cell Signalling, Vol. 16, No. 3, 01.07.1997, p. 139-145.

Research output: Contribution to journalArticle

Travers, Jeffrey B. ; Johnson, Christopher ; Clay, Keith L. ; Harrison, Kathleen ; Zekman, Tami ; Morelli, Joseph G. ; Murphy, Robert C. / Identification of sn-2 acetyl glycerophosphocholines in human keratinocytes. In: Journal of Lipid Mediators and Cell Signalling. 1997 ; Vol. 16, No. 3. pp. 139-145.
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