Identification of survival motor neuron as a transcriptional activator-binding protein

John Strasswimmer, Christian L. Lorson, David E. Breiding, Jason J. Chen, Than Le, Arthur H.M. Burghes, Elliot J. Androphy

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Spinal muscular atrophy (SMA) is an inherited neuromuscular disease characterized by specific degeneration of spinal cord anterior horn cells and subsequent muscle atrophy. Survival motor neuron (SMN), located on chromosome 5q13, is the SMA-determining gene. In the nucleus, SMN is present in large foci called gems, the function of which is not yet known, while cytoplasmic SMN has been implicated in snRNP biogenesis. In SMA patients, SMN protein levels and the number of gems generally correlate with disease severity, suggesting a critical nuclear function for SMN. In a screen for proteins associated with the nuclear transcription activator 'E2' of papillomavirus, two independent SMN cDNAs were isolated. The E2 and SMN proteins were found to associate specifically in vitro and in vivo. Expression of SMN enhanced E2-dependent transcriptional activation, and patient-derived SMN missense mutations reduced E2 gene expression. Our results demonstrate that SMN interacts with a nuclear transcription factor and imply that SMN may serve a role in regulating gene expression. These observations suggest that SMA may in part result from abnormal gene expression and that E2 may influence viral gene expression through SMN interaction.

Original languageEnglish (US)
Pages (from-to)1219-1226
Number of pages8
JournalHuman molecular genetics
Volume8
Issue number7
DOIs
StatePublished - Jul 27 1999
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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    Strasswimmer, J., Lorson, C. L., Breiding, D. E., Chen, J. J., Le, T., Burghes, A. H. M., & Androphy, E. J. (1999). Identification of survival motor neuron as a transcriptional activator-binding protein. Human molecular genetics, 8(7), 1219-1226. https://doi.org/10.1093/hmg/8.7.1219