Identification of SV40 large T-antigen-associated proteins in cardiomyocytes from transgenic mice

A. I. Daud, N. A. Lanson, W. C. Claycomb, Loren Field

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A cell line derived from transgenic mice expressing the SV40 large T- antigen oncogene in the heart was used to identify cardiomyocyte targets for T-antigen binding. A novel protein of molecular mass of 193 kDa was identified as an associated protein by virtue of its ability to be co- immunoprecipitated with multiple anti-T-antigen antibodies. Two previously described proteins, p120 and p53, were also observed to complex with T- antigen in transformed cardiomyocytes. In addition, several proteins that cross-reacted with either anti-T-antigen or anti-p53 antibodies were identified. Two of these proteins, of apparent molecular masses of 250 and 110 kDa, were only observed in cardiomyocytes. Expression of a third cross- reacting protein of a molecular mass of 180 kDa appeared to be dependent on the growth status of the cells. These proteins may be important constituents of the cardiomyocyte cell cycle, as well as potential cellular targets for myocardial regeneration.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume264
Issue number5 33-5
StatePublished - 1993

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Polyomavirus Transforming Antigens
Viral Tumor Antigens
Cardiac Myocytes
Transgenic Mice
Proteins
Oncogenes
Regeneration
Anti-Idiotypic Antibodies
Cell Cycle
Cell Line
Antibodies
Growth

Keywords

  • cardiac tumorigenesis
  • cardiomyocyte growth

ASJC Scopus subject areas

  • Physiology

Cite this

Identification of SV40 large T-antigen-associated proteins in cardiomyocytes from transgenic mice. / Daud, A. I.; Lanson, N. A.; Claycomb, W. C.; Field, Loren.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 264, No. 5 33-5, 1993.

Research output: Contribution to journalArticle

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