A fraction of culture medium of mosquito cells persistently infected with the flavivirus, Banzi virus, possesses the ability to suppress the replication of Banzi virus and contains two radiolabeled proteins of similar molecular weight. One of these proteins is presumed to be a mosquito cellular protein since it is also present in culture medium o of uninfected mosquito cells and reacts with anti-mosquito cell serum. The other protein is precipitated by anti-Banzi virus serum. Separation of these two proteins is achieved by high-voltage paper electrophoresis. The viral protein isolated from culture medium of Banzi virus persistently infected mosquito cells has the same tryptic peptides as V-1 isolated from purified Banzi virions and competes with V-1 for anti- Banzi virus serum. Treatment of mosquito cells with purified preparations of the viral protein obtained from medium of Banzi virus persistently infected mosquito cells or with V-1 isolated from Banzi virus results in the inhibition of replication of Banzi virus but not of Japanese encephalitis or Eastern equine encephalitis viruses.
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