Identification of the antiviral factor in culture medium of mosquito cells persistently infected with Banzi virus

Chao-Hung Lee, Robert H. Schloemer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A fraction of culture medium of mosquito cells persistently infected with the flavivirus, Banzi virus, possesses the ability to suppress the replication of Banzi virus and contains two radiolabeled proteins of similar molecular weight. One of these proteins is presumed to be a mosquito cellular protein since it is also present in culture medium o of uninfected mosquito cells and reacts with anti-mosquito cell serum. The other protein is precipitated by anti-Banzi virus serum. Separation of these two proteins is achieved by high-voltage paper electrophoresis. The viral protein isolated from culture medium of Banzi virus persistently infected mosquito cells has the same tryptic peptides as V-1 isolated from purified Banzi virions and competes with V-1 for anti- Banzi virus serum. Treatment of mosquito cells with purified preparations of the viral protein obtained from medium of Banzi virus persistently infected mosquito cells or with V-1 isolated from Banzi virus results in the inhibition of replication of Banzi virus but not of Japanese encephalitis or Eastern equine encephalitis viruses.

Original languageEnglish
Pages (from-to)445-454
Number of pages10
JournalVirology
Volume110
Issue number2
DOIs
StatePublished - Apr 30 1981

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Culicidae
Antiviral Agents
Culture Media
Viruses
Viral Proteins
Proteins
Japanese Encephalitis Virus
Eastern equine encephalitis virus
Serum
Paper Electrophoresis
Flavivirus
Virus Replication
Virion
Molecular Weight

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Identification of the antiviral factor in culture medium of mosquito cells persistently infected with Banzi virus. / Lee, Chao-Hung; Schloemer, Robert H.

In: Virology, Vol. 110, No. 2, 30.04.1981, p. 445-454.

Research output: Contribution to journalArticle

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