Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines.

Elliot Androphy, N. L. Hubbert, J. T. Schiller, D. R. Lowy

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Human cervical carcinoma cell lines that harbor human papillomavirus (HPV) have been reported to retain selectively and express HPV sequences which could encode viral E6 and E7 proteins. The potential importance of HPV E6 to tumors is suggested further by the observation that bovine papillomavirus (BPV) E6 can induce morphologic transformation of mouse cells in vitro. To identify HPV E6 protein, a polypeptide encoded by HPV-16 E6 was produced in a bacterial expression vector and used to raise antisera. The antisera specifically immunoprecipitated the predicted 18-kd protein in two human carcinoma cell lines known to express HPV-16 RNA and in mouse cells morphologically transformed by HPV-16 DNA. The 18-kd E6 protein was distinct from a previously identified HPV-16 E7 protein. The HPV-16 E6 antibodies were found to be type specific in that they did not recognize E6 protein in cells containing HPV-18 sequences and reacted weakly, if at all, to BPV E6 protein. The results demonstrate that human tumors containing HPV-16 DNA can express an E6 protein product. They are consistent with the hypothesis that E6 may contribute to the transformed phenotype in human cervical cancers that express this protein.

Original languageEnglish
Pages (from-to)989-992
Number of pages4
JournalEMBO Journal
Volume6
Issue number4
StatePublished - Apr 1987
Externally publishedYes

Fingerprint

Human papillomavirus 16
Cells
Carcinoma
Cell Line
Proteins
Tumors
Immune Sera
Papillomavirus E7 Proteins
Human papillomavirus 18
Human papillomavirus type 16 E6 protein
DNA
Ports and harbors
Uterine Cervical Neoplasms
Neoplasms
RNA
Phenotype
Peptides
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines. / Androphy, Elliot; Hubbert, N. L.; Schiller, J. T.; Lowy, D. R.

In: EMBO Journal, Vol. 6, No. 4, 04.1987, p. 989-992.

Research output: Contribution to journalArticle

Androphy, Elliot ; Hubbert, N. L. ; Schiller, J. T. ; Lowy, D. R. / Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines. In: EMBO Journal. 1987 ; Vol. 6, No. 4. pp. 989-992.
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