Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus

Elliot Androphy, John T. Schiller, Douglas R. Lowy

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Papillomaviruses (PV) contain several conserved genes that may encode nonstructural proteins; however, none of these predicted gene products have been identified. Papillomavirus E6 genes are retained and expressed as RNA in PV-associated human and animal carcinomas and cell lines. This suggests that the E6 gene product may be important in the maintenance of the malignant phenotype. The E6 open reading frame of the bovine papillomavirus (BPV) genome has been identified as one of two BPV genes that can independently transform mouse cells in vitro. A polypeptide encoded by this region of BPV was produced in a bacterial expression vector and used to raise antisera. The antisera specifically immunoprecipitated the predicted 15.5-kilodalton BPV E6 protein from cells transformed by the E6 gene. The E6 protein was identified in both the nuclear and membrane fractions of these transformed cells.

Original languageEnglish (US)
Pages (from-to)442-445
Number of pages4
JournalScience
Volume230
Issue number4724
StatePublished - 1985
Externally publishedYes

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Oncogenes
Genes
Proteins
Immune Sera
Nuclear Envelope
Open Reading Frames
Maintenance
Genome
RNA
Carcinoma
Phenotype
Cell Line
Peptides

ASJC Scopus subject areas

  • General

Cite this

Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus. / Androphy, Elliot; Schiller, John T.; Lowy, Douglas R.

In: Science, Vol. 230, No. 4724, 1985, p. 442-445.

Research output: Contribution to journalArticle

Androphy, Elliot ; Schiller, John T. ; Lowy, Douglas R. / Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus. In: Science. 1985 ; Vol. 230, No. 4724. pp. 442-445.
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