Identification of the site phosphorylated by casein kinase II in smooth muscle caldesmon

Alicja Wawrzynow, John H. Collins, Natalia Bogatcheva, Alexander V. Vorotnikov, Nikolai B. Gusev

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phosphorylation of avian gizzard caldesmon by casein kinase II was investigated. The enzyme incorporates about 1 mol of phosphate per mol of caldesmon. All sites of phosphorylation are located in short chymotryptic peptides with Mr, 25-27 kDa or in the short N-terminal peptide formed after cleavage of chicken gizzard caldesmon at Cys153. The primary structure of the tryptic peptide containing the main site of duck gizzard caldesmon phosphorylation is S-E-V-N-A-Q-N-X-V-A-E-D-E-T-K. where X is an unidentified residue. presumed to be phosphorserine. Thus, Ser73 is the main site phosphorylated by casein kinase II in avian gizzard caldesmon.

Original languageEnglish (US)
Pages (from-to)213-216
Number of pages4
JournalFEBS Letters
Volume289
Issue number2
DOIs
StatePublished - Sep 9 1991
Externally publishedYes

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Calmodulin-Binding Proteins
Avian Gizzard
Casein Kinase II
Smooth Muscle
Muscle
Phosphorylation
Peptides
Ducks
Chickens
Phosphates
Enzymes

Keywords

  • Caldesmon
  • Casein kinase II
  • Phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of the site phosphorylated by casein kinase II in smooth muscle caldesmon. / Wawrzynow, Alicja; Collins, John H.; Bogatcheva, Natalia; Vorotnikov, Alexander V.; Gusev, Nikolai B.

In: FEBS Letters, Vol. 289, No. 2, 09.09.1991, p. 213-216.

Research output: Contribution to journalArticle

Wawrzynow, Alicja ; Collins, John H. ; Bogatcheva, Natalia ; Vorotnikov, Alexander V. ; Gusev, Nikolai B. / Identification of the site phosphorylated by casein kinase II in smooth muscle caldesmon. In: FEBS Letters. 1991 ; Vol. 289, No. 2. pp. 213-216.
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