Identifying a series of candidate genes for mania and psychosis

A convergent functional genomics approach

Alexander Niculescu, David S. Segal, Ronald Kuczenski, Thomas Barrett, Richard L. Hauger, John R. Kelsoe

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

We have used methamphetamine treatment of rats as an animal model for psychotic mania. Specific brain regions were analyzed comprehensively for changes in gene expression using oligonucleotide GeneChip microarrays. The data was cross-matched against human genomic loci associated with either bipolar disorder or schizophrenia. Using this convergent approach, we have identified several novel candidate genes (e.g., signal transduction molecules, transcription factors, metabolic enzymes) that may be involved in the pathogenesis of mood disorders and psychosis. Furthermore, for one of these genes, G protein-coupled receptor kinase 3 (GRK3), we found by Western blot analysis evidence for decreased protein levels in a subset of patient lymphoblastoid cell lines that correlated with disease severity. Finally, the classification of these candidate genes into two prototypical categories, psychogenes and psychosis-suppressor genes, is described.

Original languageEnglish (US)
Pages (from-to)83-91
Number of pages9
JournalPhysiological Genomics
Volume2001
Issue number4
StatePublished - Feb 2001
Externally publishedYes

Fingerprint

Genomics
Bipolar Disorder
Psychotic Disorders
G-Protein-Coupled Receptor Kinase 3
Suppressor Genes
Methamphetamine
Oligonucleotide Array Sequence Analysis
Mood Disorders
Genes
Signal Transduction
Schizophrenia
Proteins
Transcription Factors
Animal Models
Western Blotting
Gene Expression
Cell Line
Brain
Enzymes
Therapeutics

Keywords

  • Amphetamine
  • Brain
  • GRK3
  • Microarrays

ASJC Scopus subject areas

  • Genetics
  • Physiology

Cite this

Niculescu, A., Segal, D. S., Kuczenski, R., Barrett, T., Hauger, R. L., & Kelsoe, J. R. (2001). Identifying a series of candidate genes for mania and psychosis: A convergent functional genomics approach. Physiological Genomics, 2001(4), 83-91.

Identifying a series of candidate genes for mania and psychosis : A convergent functional genomics approach. / Niculescu, Alexander; Segal, David S.; Kuczenski, Ronald; Barrett, Thomas; Hauger, Richard L.; Kelsoe, John R.

In: Physiological Genomics, Vol. 2001, No. 4, 02.2001, p. 83-91.

Research output: Contribution to journalArticle

Niculescu, A, Segal, DS, Kuczenski, R, Barrett, T, Hauger, RL & Kelsoe, JR 2001, 'Identifying a series of candidate genes for mania and psychosis: A convergent functional genomics approach', Physiological Genomics, vol. 2001, no. 4, pp. 83-91.
Niculescu, Alexander ; Segal, David S. ; Kuczenski, Ronald ; Barrett, Thomas ; Hauger, Richard L. ; Kelsoe, John R. / Identifying a series of candidate genes for mania and psychosis : A convergent functional genomics approach. In: Physiological Genomics. 2001 ; Vol. 2001, No. 4. pp. 83-91.
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