Identifying Genetic Variants in Adolescents With Oppositional Defiant Disorders and/or Conduct Disorders: A Brief Report

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

PROBLEM: To add to diversity in our state biobank, we explored the feasibility of collecting genetic material from adolescents with oppositional defiant disorder (ODD) and/or conduct disorder (CD) and their family members. We also preliminarily explored genetic factors associated with ODD and/or CD by comparing participant data to 1000 Genome Project data on minor allele frequencies. METHODS: Adolescents with ODD and/or CD and family members provided saliva samples for genetic testing. We evaluated five single-nucleotide polymorphisms (SNPs), respectively, in the dopamine receptor subtype D2, dopamine receptor subtype D3, dopamine beta-hydroxylase, dopamine transporter gene SLC6A3, and alpha-2-adrenergic receptor genes. Fisher's exact tests were used to examine differences in minor allele frequencies for each SNP. FINDINGS: Thirty-one viable samples were genotyped from 15 affected adolescents and 16 unaffected family members; the 60% consent rate reflected high feasibility. Compared with the 1000 Genome Project frequencies, affected adolescents had higher frequencies of the genetic variant in the dopamine receptor subtype D2 (p =.05) and dopamine beta-hydroxylase (p = 0.03), but not of the other three SNPs examined. CONCLUSIONS: Collecting genetic materials from an ethnically diverse sample of affected adolescents and their families is feasible. We offer practical suggestions to strengthen the integrity of future research studies.

Original languageEnglish (US)
Pages (from-to)154-157
Number of pages4
JournalJournal of Child and Adolescent Psychiatric Nursing
Volume29
Issue number3
DOIs
StatePublished - Aug 1 2016

Fingerprint

Attention Deficit and Disruptive Behavior Disorders
Conduct Disorder
Single Nucleotide Polymorphism
Dopamine beta-Hydroxylase
Dopamine D2 Receptors
Gene Frequency
Genes
Adrenergic alpha-2 Receptors
Genome
Dopamine D3 Receptors
Dopamine Plasma Membrane Transport Proteins
Genetic Testing
Saliva

Keywords

  • genetic variants
  • precision medicine
  • saliva samples

ASJC Scopus subject areas

  • Phychiatric Mental Health
  • Pediatrics
  • Psychiatry and Mental health

Cite this

@article{cfc07422732d488a87f000267d327077,
title = "Identifying Genetic Variants in Adolescents With Oppositional Defiant Disorders and/or Conduct Disorders: A Brief Report",
abstract = "PROBLEM: To add to diversity in our state biobank, we explored the feasibility of collecting genetic material from adolescents with oppositional defiant disorder (ODD) and/or conduct disorder (CD) and their family members. We also preliminarily explored genetic factors associated with ODD and/or CD by comparing participant data to 1000 Genome Project data on minor allele frequencies. METHODS: Adolescents with ODD and/or CD and family members provided saliva samples for genetic testing. We evaluated five single-nucleotide polymorphisms (SNPs), respectively, in the dopamine receptor subtype D2, dopamine receptor subtype D3, dopamine beta-hydroxylase, dopamine transporter gene SLC6A3, and alpha-2-adrenergic receptor genes. Fisher's exact tests were used to examine differences in minor allele frequencies for each SNP. FINDINGS: Thirty-one viable samples were genotyped from 15 affected adolescents and 16 unaffected family members; the 60{\%} consent rate reflected high feasibility. Compared with the 1000 Genome Project frequencies, affected adolescents had higher frequencies of the genetic variant in the dopamine receptor subtype D2 (p =.05) and dopamine beta-hydroxylase (p = 0.03), but not of the other three SNPs examined. CONCLUSIONS: Collecting genetic materials from an ethnically diverse sample of affected adolescents and their families is feasible. We offer practical suggestions to strengthen the integrity of future research studies.",
keywords = "genetic variants, precision medicine, saliva samples",
author = "Ukamaka Oruche and Ross, {Sydney E.} and Janet Carpenter and Jamie Renbarger",
year = "2016",
month = "8",
day = "1",
doi = "10.1111/jcap.12153",
language = "English (US)",
volume = "29",
pages = "154--157",
journal = "Journal of Child and Adolescent Psychiatric Nursing",
issn = "1073-6077",
publisher = "Nursecom Publication",
number = "3",

}

TY - JOUR

T1 - Identifying Genetic Variants in Adolescents With Oppositional Defiant Disorders and/or Conduct Disorders

T2 - A Brief Report

AU - Oruche, Ukamaka

AU - Ross, Sydney E.

AU - Carpenter, Janet

AU - Renbarger, Jamie

PY - 2016/8/1

Y1 - 2016/8/1

N2 - PROBLEM: To add to diversity in our state biobank, we explored the feasibility of collecting genetic material from adolescents with oppositional defiant disorder (ODD) and/or conduct disorder (CD) and their family members. We also preliminarily explored genetic factors associated with ODD and/or CD by comparing participant data to 1000 Genome Project data on minor allele frequencies. METHODS: Adolescents with ODD and/or CD and family members provided saliva samples for genetic testing. We evaluated five single-nucleotide polymorphisms (SNPs), respectively, in the dopamine receptor subtype D2, dopamine receptor subtype D3, dopamine beta-hydroxylase, dopamine transporter gene SLC6A3, and alpha-2-adrenergic receptor genes. Fisher's exact tests were used to examine differences in minor allele frequencies for each SNP. FINDINGS: Thirty-one viable samples were genotyped from 15 affected adolescents and 16 unaffected family members; the 60% consent rate reflected high feasibility. Compared with the 1000 Genome Project frequencies, affected adolescents had higher frequencies of the genetic variant in the dopamine receptor subtype D2 (p =.05) and dopamine beta-hydroxylase (p = 0.03), but not of the other three SNPs examined. CONCLUSIONS: Collecting genetic materials from an ethnically diverse sample of affected adolescents and their families is feasible. We offer practical suggestions to strengthen the integrity of future research studies.

AB - PROBLEM: To add to diversity in our state biobank, we explored the feasibility of collecting genetic material from adolescents with oppositional defiant disorder (ODD) and/or conduct disorder (CD) and their family members. We also preliminarily explored genetic factors associated with ODD and/or CD by comparing participant data to 1000 Genome Project data on minor allele frequencies. METHODS: Adolescents with ODD and/or CD and family members provided saliva samples for genetic testing. We evaluated five single-nucleotide polymorphisms (SNPs), respectively, in the dopamine receptor subtype D2, dopamine receptor subtype D3, dopamine beta-hydroxylase, dopamine transporter gene SLC6A3, and alpha-2-adrenergic receptor genes. Fisher's exact tests were used to examine differences in minor allele frequencies for each SNP. FINDINGS: Thirty-one viable samples were genotyped from 15 affected adolescents and 16 unaffected family members; the 60% consent rate reflected high feasibility. Compared with the 1000 Genome Project frequencies, affected adolescents had higher frequencies of the genetic variant in the dopamine receptor subtype D2 (p =.05) and dopamine beta-hydroxylase (p = 0.03), but not of the other three SNPs examined. CONCLUSIONS: Collecting genetic materials from an ethnically diverse sample of affected adolescents and their families is feasible. We offer practical suggestions to strengthen the integrity of future research studies.

KW - genetic variants

KW - precision medicine

KW - saliva samples

UR - http://www.scopus.com/inward/record.url?scp=84987837772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84987837772&partnerID=8YFLogxK

U2 - 10.1111/jcap.12153

DO - 10.1111/jcap.12153

M3 - Article

C2 - 27633436

AN - SCOPUS:84987837772

VL - 29

SP - 154

EP - 157

JO - Journal of Child and Adolescent Psychiatric Nursing

JF - Journal of Child and Adolescent Psychiatric Nursing

SN - 1073-6077

IS - 3

ER -