IFATS collection: Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: Role of canonical Wnt signaling

Gangaraju Rajashekhar, Dmitry Traktuev, William C. Roell, Brian H. Johnstone, Stephanie Merfeld-Clauss, Bruce Van Natta, Elliot Rosen, Keith L. March, Matthias Clauss

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Adipose stromal cells (ASC) are multipotential mesenchymal progenitor cells that are readily induced to undergo adipogenic differentiation, and we have recently demonstrated them to have functional and phenotypic overlap with pericytes lining microvessels in adipose tissues. In this study we addressed the hypothesis that modulation of ASC fate within this perivascular niche can occur via interaction with endothelial cells (EC), which serve to modulate the adipogenic potential of ASC. To this end, we investigated contact as well as paracrine effects of EC on ASC adipogenesis, in two-dimensional coculture and via conditioned medium and analyzed mutual gene expression changes by real-time reverse transcription polymerase chain reaction (PCR). A significant decrease in adipogenic differentiation was observed in ASC when they were cocultured with EC but not control fibroblasts. This endothelial cell-specific effect was accompanied by increased expression of factors involved in Wnt signaling, most prominently Wnt1, Wnt4, and Wnt10a, which are well-known inhibitors of adipogenesis. Suppression of Wnt1 but not Wnt 10a or scrambled control short interfering RNA in cocultures partially reversed the endothelial cell effect, thus increasing adipogenic differentiation, suggesting a plausible role of Wnt1 ligand in modulation of adipogenesis by the vasculature. Furthermore, addition of recombinant Wnt ligand or the Wnt signaling agonist inhibited adipogenic differentiation of ASC in the absence of EC. In conclusion, these data define the relationship in adipose tissue between ASC and EC in the perivascular niche, in which the latter act to repress adipogenesis, thereby stabilizing vasculature. It is tempting to speculate that abnormal endothelial function may be associated with pathologic derepression of adipogenesis.

Original languageEnglish
Pages (from-to)2674-2681
Number of pages8
JournalStem Cells
Volume26
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

Paracrine Communication
Stromal Cells
Cell Communication
Adipogenesis
Cell Differentiation
Endothelial Cells
Coculture Techniques
Adipose Tissue
Ligands
Pericytes
Conditioned Culture Medium
Microvessels
Mesenchymal Stromal Cells
Small Interfering RNA
Reverse Transcription
Fibroblasts
Gene Expression
Polymerase Chain Reaction

Keywords

  • Adipogenesis
  • Adipose stromal cells
  • Adult stem cells
  • Differentiation
  • Short interfering RNA
  • Wnt1

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

IFATS collection : Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: Role of canonical Wnt signaling. / Rajashekhar, Gangaraju; Traktuev, Dmitry; Roell, William C.; Johnstone, Brian H.; Merfeld-Clauss, Stephanie; Van Natta, Bruce; Rosen, Elliot; March, Keith L.; Clauss, Matthias.

In: Stem Cells, Vol. 26, No. 10, 10.2008, p. 2674-2681.

Research output: Contribution to journalArticle

Rajashekhar, Gangaraju ; Traktuev, Dmitry ; Roell, William C. ; Johnstone, Brian H. ; Merfeld-Clauss, Stephanie ; Van Natta, Bruce ; Rosen, Elliot ; March, Keith L. ; Clauss, Matthias. / IFATS collection : Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: Role of canonical Wnt signaling. In: Stem Cells. 2008 ; Vol. 26, No. 10. pp. 2674-2681.
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