IGFBP-2 is increased in pig vitreous by retinal ischemia

D. P. Bingaman, R. P. Danis, Y. Yang, W. M. Lee, B. Ladd, M. Grant

Research output: Contribution to journalArticle

Abstract

PURPOSE: Components of the IGF system have been found to be modulated in the eyes of human diabetics and animal models of experimental hyperhexosemia. We sought to determine if the IGF system is altered from normal in the domestic pig model of preretinal NV induced by ischemia, with and without exogenous IGF-1 administration. METHODS: 25 pigs were treated with bilateral branch retinal vein occlusion, 16 pigs were injected intravitreally with 600ug of hrIGF-1 in one eye, one week post-BVO (the control eye received BSA) Pigs were sacrificed ii groups of four at 1,4,12, and 24 weeks post-injection. The 9 additional pigs were eft untreated and sacrificed in groups of 3 at 3, 7, and 28 days post-BVO. All vitreous samples were subjected to western ligand and immunoblotting. Each sample was studied for total protein MW spectrum with enhanced gold staining, all IGFBPs were labeled with [125]I-IGF-II: and IGFBPs were specifically identified with rabbit, anti-human IGFBP-2, 3, and 4 antibodies RESULTS: All eyes showed a labeled band consistent with IGFRP-2 (BP-2), which was confirmed by immunoblotting. BP-2 levels were very high from 72 hrs post-BVO and were maintained at an eleva ed level throughout the study period compared to normals. Eyes injected with IGF-1 demonstrated increased BP-2 levels vs the control eyes in most groups. CONCLUSIONS: This is the first report demonstrating the presence of BP-2 in the pig vitreous and its modulation by experimental retinal ischemia (±exogenously delivered IGF-1). Because elevated BP-2 levels have also been found in the vitreous of humans with PDR, the IGF system may play a significant role in the pathophysiology of diabetic complications.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Insulin-Like Growth Factor Binding Protein 2
Swine
Ischemia
Insulin-Like Growth Factor I
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor Binding Protein 4
Retinal Vein Occlusion
Sus scrofa
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor II
Diabetes Complications
Immunoblotting
Gold
Animal Models
Western Blotting
Staining and Labeling
Rabbits
Ligands
Injections
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Bingaman, D. P., Danis, R. P., Yang, Y., Lee, W. M., Ladd, B., & Grant, M. (1996). IGFBP-2 is increased in pig vitreous by retinal ischemia. Investigative Ophthalmology and Visual Science, 37(3).

IGFBP-2 is increased in pig vitreous by retinal ischemia. / Bingaman, D. P.; Danis, R. P.; Yang, Y.; Lee, W. M.; Ladd, B.; Grant, M.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Bingaman, DP, Danis, RP, Yang, Y, Lee, WM, Ladd, B & Grant, M 1996, 'IGFBP-2 is increased in pig vitreous by retinal ischemia', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Bingaman DP, Danis RP, Yang Y, Lee WM, Ladd B, Grant M. IGFBP-2 is increased in pig vitreous by retinal ischemia. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Bingaman, D. P. ; Danis, R. P. ; Yang, Y. ; Lee, W. M. ; Ladd, B. ; Grant, M. / IGFBP-2 is increased in pig vitreous by retinal ischemia. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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abstract = "PURPOSE: Components of the IGF system have been found to be modulated in the eyes of human diabetics and animal models of experimental hyperhexosemia. We sought to determine if the IGF system is altered from normal in the domestic pig model of preretinal NV induced by ischemia, with and without exogenous IGF-1 administration. METHODS: 25 pigs were treated with bilateral branch retinal vein occlusion, 16 pigs were injected intravitreally with 600ug of hrIGF-1 in one eye, one week post-BVO (the control eye received BSA) Pigs were sacrificed ii groups of four at 1,4,12, and 24 weeks post-injection. The 9 additional pigs were eft untreated and sacrificed in groups of 3 at 3, 7, and 28 days post-BVO. All vitreous samples were subjected to western ligand and immunoblotting. Each sample was studied for total protein MW spectrum with enhanced gold staining, all IGFBPs were labeled with [125]I-IGF-II: and IGFBPs were specifically identified with rabbit, anti-human IGFBP-2, 3, and 4 antibodies RESULTS: All eyes showed a labeled band consistent with IGFRP-2 (BP-2), which was confirmed by immunoblotting. BP-2 levels were very high from 72 hrs post-BVO and were maintained at an eleva ed level throughout the study period compared to normals. Eyes injected with IGF-1 demonstrated increased BP-2 levels vs the control eyes in most groups. CONCLUSIONS: This is the first report demonstrating the presence of BP-2 in the pig vitreous and its modulation by experimental retinal ischemia (±exogenously delivered IGF-1). Because elevated BP-2 levels have also been found in the vitreous of humans with PDR, the IGF system may play a significant role in the pathophysiology of diabetic complications.",
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N2 - PURPOSE: Components of the IGF system have been found to be modulated in the eyes of human diabetics and animal models of experimental hyperhexosemia. We sought to determine if the IGF system is altered from normal in the domestic pig model of preretinal NV induced by ischemia, with and without exogenous IGF-1 administration. METHODS: 25 pigs were treated with bilateral branch retinal vein occlusion, 16 pigs were injected intravitreally with 600ug of hrIGF-1 in one eye, one week post-BVO (the control eye received BSA) Pigs were sacrificed ii groups of four at 1,4,12, and 24 weeks post-injection. The 9 additional pigs were eft untreated and sacrificed in groups of 3 at 3, 7, and 28 days post-BVO. All vitreous samples were subjected to western ligand and immunoblotting. Each sample was studied for total protein MW spectrum with enhanced gold staining, all IGFBPs were labeled with [125]I-IGF-II: and IGFBPs were specifically identified with rabbit, anti-human IGFBP-2, 3, and 4 antibodies RESULTS: All eyes showed a labeled band consistent with IGFRP-2 (BP-2), which was confirmed by immunoblotting. BP-2 levels were very high from 72 hrs post-BVO and were maintained at an eleva ed level throughout the study period compared to normals. Eyes injected with IGF-1 demonstrated increased BP-2 levels vs the control eyes in most groups. CONCLUSIONS: This is the first report demonstrating the presence of BP-2 in the pig vitreous and its modulation by experimental retinal ischemia (±exogenously delivered IGF-1). Because elevated BP-2 levels have also been found in the vitreous of humans with PDR, the IGF system may play a significant role in the pathophysiology of diabetic complications.

AB - PURPOSE: Components of the IGF system have been found to be modulated in the eyes of human diabetics and animal models of experimental hyperhexosemia. We sought to determine if the IGF system is altered from normal in the domestic pig model of preretinal NV induced by ischemia, with and without exogenous IGF-1 administration. METHODS: 25 pigs were treated with bilateral branch retinal vein occlusion, 16 pigs were injected intravitreally with 600ug of hrIGF-1 in one eye, one week post-BVO (the control eye received BSA) Pigs were sacrificed ii groups of four at 1,4,12, and 24 weeks post-injection. The 9 additional pigs were eft untreated and sacrificed in groups of 3 at 3, 7, and 28 days post-BVO. All vitreous samples were subjected to western ligand and immunoblotting. Each sample was studied for total protein MW spectrum with enhanced gold staining, all IGFBPs were labeled with [125]I-IGF-II: and IGFBPs were specifically identified with rabbit, anti-human IGFBP-2, 3, and 4 antibodies RESULTS: All eyes showed a labeled band consistent with IGFRP-2 (BP-2), which was confirmed by immunoblotting. BP-2 levels were very high from 72 hrs post-BVO and were maintained at an eleva ed level throughout the study period compared to normals. Eyes injected with IGF-1 demonstrated increased BP-2 levels vs the control eyes in most groups. CONCLUSIONS: This is the first report demonstrating the presence of BP-2 in the pig vitreous and its modulation by experimental retinal ischemia (±exogenously delivered IGF-1). Because elevated BP-2 levels have also been found in the vitreous of humans with PDR, the IGF system may play a significant role in the pathophysiology of diabetic complications.

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