IL-1 β activates C/EBP-β and δ in human enterocytes through a mitogen-activated protein kinase signaling pathway

Eric S. Hungness, Timothy A. Pritts, Guang Ju Luo, Dan D. Hershko, Bruce Robb, Per Olof Hasselgren

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The enterocyte is an active participant in the inflammatory and metabolic response to sepsis, endotoxemia and other critical illnesses and is the site for cytokine and acute phase protein production in these conditions. The role of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors in the response to inflammatory stimuli in the enterocyte is not well understood. In the present study, we treated Caco-2 cells with IL-1 β and determined C/EBP DNA binding activity by electrophoretic mobility shift assay. The involvement of the α, β, and δ isoforms was determined by supershift analysis and Western blot analysis of proteins from the nuclear fraction. The role of the mitogen-activated protein kinase (MAPK) signaling pathway was assessed by treating cells with the MAPK inhibitor PD-98059. Treatment of the Caco-2 cells with IL-1 β resulted in increased CCAAT/enhancer binding protein DNA binding activity. Supershift analysis and Western blotting indicated that this response to IL-1 β mainly reflected the δ isoform, and to a lesser degree the β isoform. Treatment of the cells with PD-98059 inhibited the IL-1 β-induced increase in β and δ activity. The results suggest that members of the CCAAT/enhancer binding protein family of transcription factors are activated in enterocytes during inflammatory conditions characterized by high levels of IL-1 β.

Original languageEnglish (US)
Pages (from-to)382-395
Number of pages14
JournalInternational Journal of Biochemistry and Cell Biology
Volume34
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

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CCAAT-Enhancer-Binding Proteins
Enterocytes
Mitogen-Activated Protein Kinases
Interleukin-1
Protein Isoforms
Caco-2 Cells
Protein Binding
Transcription Factors
Western Blotting
Electrophoretic mobility
Endotoxemia
Acute-Phase Proteins
DNA
Electrophoretic Mobility Shift Assay
Protein Kinase Inhibitors
Nuclear Proteins
Critical Illness
Assays
Sepsis
Cells

Keywords

  • C/EBP
  • Enterocyte
  • Human
  • IL-1
  • MAP Kinase
  • Sepsis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

IL-1 β activates C/EBP-β and δ in human enterocytes through a mitogen-activated protein kinase signaling pathway. / Hungness, Eric S.; Pritts, Timothy A.; Luo, Guang Ju; Hershko, Dan D.; Robb, Bruce; Hasselgren, Per Olof.

In: International Journal of Biochemistry and Cell Biology, Vol. 34, No. 4, 2002, p. 382-395.

Research output: Contribution to journalArticle

Hungness, Eric S. ; Pritts, Timothy A. ; Luo, Guang Ju ; Hershko, Dan D. ; Robb, Bruce ; Hasselgren, Per Olof. / IL-1 β activates C/EBP-β and δ in human enterocytes through a mitogen-activated protein kinase signaling pathway. In: International Journal of Biochemistry and Cell Biology. 2002 ; Vol. 34, No. 4. pp. 382-395.
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