IL-4 Induces Differentiation and Expansion of Th2 Cytokine-Producing Eosinophils

Luqiu Chen, Kristy A. Grabowski, Jun Ping Xin, John Coleman, Zan Huang, Baltazar Espiritu, Serhan Alkan, H. Bill Xie, Yuechun Zhu, Fletcher A. White, John Clancy, Hua Huang

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Innate effector cells that produce Th2-type cytokines are critical in Th2 cell-mediated immune responses, However, it is not known how these cells acquire the ability to produce Th2 cytokines. IL-4 is a potent inducer that directs differentiation of naive CD4+ T cells into CD4+ Th2 effector cells. To determine whether IL-4 can induce differentiation and expansion of Th2 cytokine-producing innate cells, we used mice whose il-4 gene was replaced by a knock-in green fluorescence protein (gfp) gene. We found that, directly ex vivo, IL-4 increased the number of GFP+ cells in the airway and the lung tissue in an Ag-specific manner. The majority of GFP + cells were eosinophils, suggesting that IL-4 plays a pivotal role in expanding IL-4-producing eosinophils in vivo. IL-4-producing eosinophils showed some unique features compared with IL-4-producing CD4+ T cells. They exhibited biallelic expression of the il-4 gene when stimulated and were more dominant IL-4- and IL-5-producing cells. Furthermore, we show that IL-4 drove bone marrow progenitor cells to differentiate into Th2 cytokine-producing eosinophils in vitro. These results strongly suggest IL-4 is a potent factor in directing bone marrow progenitor cells to differentiate into Th2 cytokine-producing eosinophils.

Original languageEnglish (US)
Pages (from-to)2059-2066
Number of pages8
JournalJournal of Immunology
Volume172
Issue number4
DOIs
StatePublished - Feb 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Chen, L., Grabowski, K. A., Xin, J. P., Coleman, J., Huang, Z., Espiritu, B., Alkan, S., Xie, H. B., Zhu, Y., White, F. A., Clancy, J., & Huang, H. (2004). IL-4 Induces Differentiation and Expansion of Th2 Cytokine-Producing Eosinophils. Journal of Immunology, 172(4), 2059-2066. https://doi.org/10.4049/jimmunol.172.4.2059