IL-4-stimulated NF-κB activity is required for Stat6 DNA binding

Vivian T. Thieu, Evelyn T. Nguyen, Brian P. McCarthy, Heather A. Bruns, Reuben Kapur, Cheong Hee Chang, Mark Kaplan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

IL-4 is a critical cytokine in the regulation of immune responses. In B lymphocytes, IL-4 signaling promotes the Stat6-dependent cell surface expression of several proteins including MHC Class II and CD86. However, the requirement for other transcription factors in IL-4-induced B cell gene expression has not been studied extensively. Here, we show that IL-4 induces NF-κB p100 processing to NF-κB p52 in B cells but not in T cells or macrophages. IL-4 induced NF-κB p52 production requires PI-3K activity and correlates with IκB kinase phosphorylation and TNF receptor-associated factor 3 degradation. Blocking NF-κB activity eliminates IL-4-stimulated gene expression in B cells by reducing IL-4-induced DNA binding but not phosphorylation or nuclear localization of Stat6. These results describe a novel role for NF-κB in IL-4-induced signaling and gene expression.

Original languageEnglish
Pages (from-to)370-379
Number of pages10
JournalJournal of Leukocyte Biology
Volume82
Issue number2
DOIs
StatePublished - Aug 1 2007

Fingerprint

Interleukin-4
DNA
B-Lymphocytes
Gene Expression
TNF Receptor-Associated Factor 3
Phosphorylation
Phosphatidylinositol 3-Kinases
Transcription Factors
Phosphotransferases
Macrophages
Cytokines
T-Lymphocytes
Proteins

Keywords

  • Cytokines
  • Gene regulation
  • p100 processing
  • Transcription factors

ASJC Scopus subject areas

  • Cell Biology

Cite this

IL-4-stimulated NF-κB activity is required for Stat6 DNA binding. / Thieu, Vivian T.; Nguyen, Evelyn T.; McCarthy, Brian P.; Bruns, Heather A.; Kapur, Reuben; Chang, Cheong Hee; Kaplan, Mark.

In: Journal of Leukocyte Biology, Vol. 82, No. 2, 01.08.2007, p. 370-379.

Research output: Contribution to journalArticle

Thieu, Vivian T. ; Nguyen, Evelyn T. ; McCarthy, Brian P. ; Bruns, Heather A. ; Kapur, Reuben ; Chang, Cheong Hee ; Kaplan, Mark. / IL-4-stimulated NF-κB activity is required for Stat6 DNA binding. In: Journal of Leukocyte Biology. 2007 ; Vol. 82, No. 2. pp. 370-379.
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