IL-6 stimulates osteoclast-like multinucleated cell formation in long term human marrow cultures by inducing IL-1 release

Noriyoshi Kurihara, Donald Bertolini, Toshio Suda, Yukio Akiyama, G. David Roodman

Research output: Contribution to journalArticle

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Abstract

IL-6 enhances the differentiation of pluripotent hematopoietic stem cells but predominantly affects the differentiation of hematopoietic cells in the granulocyte-macrophage lineage. We have previously shown that multinucleated cells (MNC) with many features of the osteoclast phenotype form in long term human marrow cultures. Addition of rhIL-6 (10 to 100 pg/ml) to these cultures significantly increased MNC formation, with >80% of the MNC expressing an Ag that cross-reacts with the mAb 23c6. This antibody preferentially binds to osteoclasts. rhIL-6 did not enhance MNC formation in marrow cultures treated with 1,25 dihydroxyvitamin D3, a potent stimulator of MNC formation, but significantly increased the percentage of MNC that cross-reacted with the 23c6 mAb. Addition of antihuman IL-1 to cultures treated with rhIL-6 totally inhibited the increase in MNC formation stimulated by rhIL-6. In contrast, anti-IL-1 did not affect MNC formation stimulated by 1,25 dihydroxyvitamin D3. Further, conditioned media from marrow cultures exposed to rhIL-6 contained elevated levels of IL-1β (500 pg/ml compared to 23 pg/ml in control cultures 15 h after IL-6 addition). These results suggest that the capacity of rhIL-6 to stimulate formation of MNC which cross-react with 23c6 is mediated by induction of release of IL-1β.

Original languageEnglish (US)
Pages (from-to)4226-4230
Number of pages5
JournalJournal of Immunology
Volume144
Issue number11
StatePublished - Jun 1 1990
Externally publishedYes

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Osteoclasts
Interleukin-1
Interleukin-6
Bone Marrow
Calcitriol
Pluripotent Stem Cells
Conditioned Culture Medium
Hematopoietic Stem Cells
Granulocytes
Cell Differentiation
Macrophages
Phenotype
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

IL-6 stimulates osteoclast-like multinucleated cell formation in long term human marrow cultures by inducing IL-1 release. / Kurihara, Noriyoshi; Bertolini, Donald; Suda, Toshio; Akiyama, Yukio; Roodman, G. David.

In: Journal of Immunology, Vol. 144, No. 11, 01.06.1990, p. 4226-4230.

Research output: Contribution to journalArticle

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AU - Roodman, G. David

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