Imaging and quantitative analysis of atherosclerotic lesions by CARS-based multimodal nonlinear optical microscopy

Han Wei Wang, Ingeborg M. Langohr, Michael Sturek, Ji Xin Cheng

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

OBJECTIVE-: The purpose of this study was to assess the ability of label-free multimodal nonlinear optical (NLO) microscopy to characterize, and thus enable quantitative in situ analyses of, different atherosclerotic lesion types, according to the original scheme suggested by the AHA Committee. METHODS AND RESULTS-: Iliac arteries were taken from 24 male Ossabaw pigs divided into lean control and metabolic syndrome groups and were imaged by multimodal NLO microscopy where sum-frequency generation (SFG) and 2-photon excitation fluorescence (TPEF) were integrated on a coherent anti-Stokes Raman scattering (CARS) microscope platform. Foam cells, lipid deposits, matrices, and fibrous caps were visualized with submicron 3D resolution. Starting from the adaptive intimal thickening in the initial stage to the fibrous atheroma or mineralization in the advanced stages, lesions were visualized without labels. Histological staining of each lesion confirmed the lesion stages. Lipid and collagen contents were quantitatively analyzed based on the CARS and SFG signals. Lipid accumulation in thickened intima culminated in type IV whereas the highest collagen deposition was found in Type V lesions. Luminal CARS imaging showed the capability of viewing the location of superficial foam cells that indicate relatively active locus in a lesion artery. CONCLUSIONS-: We have demonstrated the capability of CARS-based multimodal NLO microscopy to interrogate different stages of lesion development with subcellular detail to permit quantitative analysis of lipid and collagen contents.

Original languageEnglish
Pages (from-to)1342-1348
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume29
Issue number9
DOIs
StatePublished - Sep 2009

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Raman Spectrum Analysis
Microscopy
Lipids
Foam Cells
Collagen
Tunica Intima
Iliac Artery
Atherosclerotic Plaques
Photons
Swine
Arteries
Fluorescence
Staining and Labeling

Keywords

  • Atherosclerosis
  • Coherent antistokes Raman scattering
  • Histopathology
  • Multimodal nonlinear optical microscopy
  • Ossabaw miniature swine

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Imaging and quantitative analysis of atherosclerotic lesions by CARS-based multimodal nonlinear optical microscopy. / Wang, Han Wei; Langohr, Ingeborg M.; Sturek, Michael; Cheng, Ji Xin.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 29, No. 9, 09.2009, p. 1342-1348.

Research output: Contribution to journalArticle

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