Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy

Han Wei Wang, Vlad Simianu, Mattew J. Locker, Michael Sturek, Ji Xin Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

By integrating sum-frequency generation (SFG), and two-photon excitation fluorescence (TPEF) on a coherent antiStokes Raman scattering (CARS) microscope platform, multimodal nonlinear optical (NLO) imaging of arteries and atherosclerotic lesions was demonstrated. CARS signals arising from CH 2-rich membranes allowed visualization of endothelial cells and smooth muscle cells in a carotid artery. Additionally, CARS microscopy allowed vibrational imaging of elastin and collagen fibrils which are rich in CH 2 bonds in their cross-linking residues. The extracellular matrix organization was further confirmed by TPEF signals arising from elastin's autofluorescence and SFG signals arising from collagen fibrils' non-centrosymmetric structure. The system is capable of identifying different atherosclerotic lesion stages with sub-cellular resolution. The stages of atherosclerosis, such as macrophage infiltration, lipid-laden foam cell accumulation, extracellular lipid distribution, fibrous tissue deposition, plaque establishment, and formation of other complicated lesions could be viewed by our multimodal CARS microscope. Collagen percentages in the region adjacent to coronary artery stents were resolved. High correlation between NLO and histology imaging evidenced the validity of the NLO imaging. The capability of imaging significant components of an arterial wall and distinctive stages of atherosclerosis in a label-free manner suggests the potential application of multimodal nonlinear optical microscopy to monitor the onset and progression of arterial diseases.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6860
DOIs
StatePublished - 2008
EventMultiphoton Microscopy in the Biomedical Sciences VIII - San Jose, CA, United States
Duration: Jan 20 2008Jan 22 2008

Other

OtherMultiphoton Microscopy in the Biomedical Sciences VIII
CountryUnited States
CitySan Jose, CA
Period1/20/081/22/08

Fingerprint

Coherent scattering
Optical microscopy
Raman scattering
Imaging techniques
Collagen
Elastin
Lipids
Microscopes
Photons
Fluorescence
Histology
Stents
Macrophages
Endothelial cells
Infiltration
Muscle
Foams
Labels
Microscopic examination
Visualization

Keywords

  • Atherosclerosis
  • CARS
  • Collagen
  • Foam cell
  • Multimodal

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wang, H. W., Simianu, V., Locker, M. J., Sturek, M., & Cheng, J. X. (2008). Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6860). [68600W] https://doi.org/10.1117/12.763604

Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy. / Wang, Han Wei; Simianu, Vlad; Locker, Mattew J.; Sturek, Michael; Cheng, Ji Xin.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6860 2008. 68600W.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, HW, Simianu, V, Locker, MJ, Sturek, M & Cheng, JX 2008, Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6860, 68600W, Multiphoton Microscopy in the Biomedical Sciences VIII, San Jose, CA, United States, 1/20/08. https://doi.org/10.1117/12.763604
Wang HW, Simianu V, Locker MJ, Sturek M, Cheng JX. Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6860. 2008. 68600W https://doi.org/10.1117/12.763604
Wang, Han Wei ; Simianu, Vlad ; Locker, Mattew J. ; Sturek, Michael ; Cheng, Ji Xin. / Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6860 2008.
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