Imaging molecular interactions in living cells

Richard Day, Fred Schaufele

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Hormones integrate the activities of their target cells through receptor-modulated cascades of protein interactions that ultimately lead to changes in cellular function. Understanding how the cell assembles these signaling protein complexes is critically important to unraveling disease processes, and to the design of therapeutic strategies. Recent advances in live-cell imaging technologies, combined with the use of genetically encoded fluorescent proteins, now allow the assembly of these signaling protein complexes to be tracked within the organized microenvironment of the living cell. Here, we review some of the recent developments in the application of imaging techniques to measure the dynamic behavior, colocalization, and spatial relationships between proteins in living cells. Where possible, we discuss the application of these different approaches in the context of hormone regulation of nuclear receptor localization, mobility, and interactions in different subcellular compartments. We discuss measurements that define the spatial relationships and dynamics between proteins in living cells including fluorescence colocalization, fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, fluorescence resonance energy transfer microscopy, and fluorescence lifetime imaging microscopy. These live-cell imaging tools provide an important complement to biochemical and structural biology studies, extending the analysis of protein-protein interactions, protein conformational changes, and the behavior of signaling molecules to their natural environment within the intact cell.

Original languageEnglish (US)
Pages (from-to)1675-1686
Number of pages12
JournalMolecular Endocrinology
Volume19
Issue number7
DOIs
StatePublished - Jul 2005
Externally publishedYes

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Molecular Imaging
Proteins
Microscopy
Fluorescence Recovery After Photobleaching
Cellular Microenvironment
Fluorescence Resonance Energy Transfer
Optical Imaging
Fluorescence Spectrometry
Cytoplasmic and Nuclear Receptors
Fluorescence
Hormones
Technology

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Imaging molecular interactions in living cells. / Day, Richard; Schaufele, Fred.

In: Molecular Endocrinology, Vol. 19, No. 7, 07.2005, p. 1675-1686.

Research output: Contribution to journalArticle

Day, Richard ; Schaufele, Fred. / Imaging molecular interactions in living cells. In: Molecular Endocrinology. 2005 ; Vol. 19, No. 7. pp. 1675-1686.
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