Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Xiaojing Lin, Tingbao Zhao, Wenhui Xiong, Shaonan Wen, Xiaoming Jin, Xiao-Ming Xu

Research output: Contribution to journalArticle

Abstract

The mammalian brain exhibits marked symmetry across the sagittal plane. However, detailed description of neural dynamics in symmetric brain regions in adult mammalian animals remains elusive. In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy. This method enables recordings and quantifications of neural activity in bilateral mouse brain regions one at a time in the same experiment for a prolonged period in vivo. Key aspects of this method, which can be completed within an hour, include minimally invasive surgery procedures for creating dual optical windows, and the use of 2P imaging. Although we only demonstrate the technique in the S1 area, the method can be applied to other regions of the living brain facilitating the elucidation of structural and functional complexities of brain neural networks.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number143
DOIs
StatePublished - Jan 7 2019

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Somatosensory Cortex
Transgenic Mice
Brain
Imaging techniques
Photons
Minimally Invasive Surgical Procedures
Surgery
Microscopy
Calcium
Microscopic examination
Animals
Neural networks
Experiments

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice. / Lin, Xiaojing; Zhao, Tingbao; Xiong, Wenhui; Wen, Shaonan; Jin, Xiaoming; Xu, Xiao-Ming.

In: Journal of visualized experiments : JoVE, No. 143, 07.01.2019.

Research output: Contribution to journalArticle

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