Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement

Laurel M. Burk, Kohan Wang, Eunice Kang, Mauricio Rojas, Monte Willis, Yueh Z. Lee, Jianping Lu, Otto Zhou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We demonstrate the application of our cardiac- and respiratory-gated carbon nanotube (CNT) micro-CT system by evaluating murine myocardial infarction models with a delayed contrast enhancement technique. Myocardial infarction was induced in 8 wild-type male mice. The ischemia reperfusion model was achieved by surgical occlusion of the LAD artery for 30 minutes followed by 24 hours of reperfusion. Free-breathing subjects were anesthetized with isoflurane during imaging. Respiratory and cardiac signals were monitored externally to gate the scan. Micro-CT data was obtained at 50kV, 3mA cathode current for 15ms per projection. All images were acquired during end exhalation at either 0msec or 55msec after the R-wave (diastole or systole, respectively). Following administration of Omnipaque 300mgI/mL at 0.1ml/5g, images were obtained at 0msec after the R-wave. Fenestra VC was then administered at a 0.1ml/5g dose, followed by images 0 and 55msec after the R-wave. Hearts were then harvested, sliced 1mm thick and stained with TTC. All animals survived surgery and imaging; all demonstrated obvious delayed contrast enhancement in the left ventricular wall in Omnipaque images. Fenestra VC revealed cardiac functional changes quantified by low ejection fractions. All subjects demonstrated areas of myocardial infarct in the LAD distribution on both TTC staining and micro-CT imaging. CNT enabled gated cardiac micro-CT imaging demonstrates the ability to consistently identify areas of myocardial infarct in mice, providing a powerful tool for the study of cardiovascular biology. Further work is ongoing to streamline the imaging protocol and perform more quantitative analysis of the images.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2011
Subtitle of host publicationBiomedical Applications in Molecular, Structural, and Functional Imaging
DOIs
StatePublished - May 16 2011
Externally publishedYes
EventMedical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging - Lake Buena Vista, FL, United States
Duration: Feb 13 2011Feb 16 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7965
ISSN (Print)1605-7422

Conference

ConferenceMedical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging
CountryUnited States
CityLake Buena Vista, FL
Period2/13/112/16/11

Fingerprint

myocardial infarction
Carbon Nanotubes
Tomography
Carbon nanotubes
tomography
carbon nanotubes
Myocardial Infarction
Iohexol
Imaging techniques
augmentation
Reperfusion
mice
Exhalation
diastole
systole
Diastole
Systole
Isoflurane
exhalation
ischemia

Keywords

  • Carbon nanotube
  • Computed tomography
  • Gated imaging
  • Micro-CT
  • Murine model
  • Myocardial infarct

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Burk, L. M., Wang, K., Kang, E., Rojas, M., Willis, M., Lee, Y. Z., ... Zhou, O. (2011). Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement. In Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging [79651N] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7965). https://doi.org/10.1117/12.878168

Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement. / Burk, Laurel M.; Wang, Kohan; Kang, Eunice; Rojas, Mauricio; Willis, Monte; Lee, Yueh Z.; Lu, Jianping; Zhou, Otto.

Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging. 2011. 79651N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7965).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Burk, LM, Wang, K, Kang, E, Rojas, M, Willis, M, Lee, YZ, Lu, J & Zhou, O 2011, Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement. in Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging., 79651N, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, Lake Buena Vista, FL, United States, 2/13/11. https://doi.org/10.1117/12.878168
Burk LM, Wang K, Kang E, Rojas M, Willis M, Lee YZ et al. Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement. In Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging. 2011. 79651N. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.878168
Burk, Laurel M. ; Wang, Kohan ; Kang, Eunice ; Rojas, Mauricio ; Willis, Monte ; Lee, Yueh Z. ; Lu, Jianping ; Zhou, Otto. / Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement. Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging. 2011. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{12c00c3e8e4a4ebf9b54ca717e4f1565,
title = "Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement",
abstract = "We demonstrate the application of our cardiac- and respiratory-gated carbon nanotube (CNT) micro-CT system by evaluating murine myocardial infarction models with a delayed contrast enhancement technique. Myocardial infarction was induced in 8 wild-type male mice. The ischemia reperfusion model was achieved by surgical occlusion of the LAD artery for 30 minutes followed by 24 hours of reperfusion. Free-breathing subjects were anesthetized with isoflurane during imaging. Respiratory and cardiac signals were monitored externally to gate the scan. Micro-CT data was obtained at 50kV, 3mA cathode current for 15ms per projection. All images were acquired during end exhalation at either 0msec or 55msec after the R-wave (diastole or systole, respectively). Following administration of Omnipaque 300mgI/mL at 0.1ml/5g, images were obtained at 0msec after the R-wave. Fenestra VC was then administered at a 0.1ml/5g dose, followed by images 0 and 55msec after the R-wave. Hearts were then harvested, sliced 1mm thick and stained with TTC. All animals survived surgery and imaging; all demonstrated obvious delayed contrast enhancement in the left ventricular wall in Omnipaque images. Fenestra VC revealed cardiac functional changes quantified by low ejection fractions. All subjects demonstrated areas of myocardial infarct in the LAD distribution on both TTC staining and micro-CT imaging. CNT enabled gated cardiac micro-CT imaging demonstrates the ability to consistently identify areas of myocardial infarct in mice, providing a powerful tool for the study of cardiovascular biology. Further work is ongoing to streamline the imaging protocol and perform more quantitative analysis of the images.",
keywords = "Carbon nanotube, Computed tomography, Gated imaging, Micro-CT, Murine model, Myocardial infarct",
author = "Burk, {Laurel M.} and Kohan Wang and Eunice Kang and Mauricio Rojas and Monte Willis and Lee, {Yueh Z.} and Jianping Lu and Otto Zhou",
year = "2011",
month = "5",
day = "16",
doi = "10.1117/12.878168",
language = "English (US)",
isbn = "9780819485076",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Medical Imaging 2011",

}

TY - GEN

T1 - Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement

AU - Burk, Laurel M.

AU - Wang, Kohan

AU - Kang, Eunice

AU - Rojas, Mauricio

AU - Willis, Monte

AU - Lee, Yueh Z.

AU - Lu, Jianping

AU - Zhou, Otto

PY - 2011/5/16

Y1 - 2011/5/16

N2 - We demonstrate the application of our cardiac- and respiratory-gated carbon nanotube (CNT) micro-CT system by evaluating murine myocardial infarction models with a delayed contrast enhancement technique. Myocardial infarction was induced in 8 wild-type male mice. The ischemia reperfusion model was achieved by surgical occlusion of the LAD artery for 30 minutes followed by 24 hours of reperfusion. Free-breathing subjects were anesthetized with isoflurane during imaging. Respiratory and cardiac signals were monitored externally to gate the scan. Micro-CT data was obtained at 50kV, 3mA cathode current for 15ms per projection. All images were acquired during end exhalation at either 0msec or 55msec after the R-wave (diastole or systole, respectively). Following administration of Omnipaque 300mgI/mL at 0.1ml/5g, images were obtained at 0msec after the R-wave. Fenestra VC was then administered at a 0.1ml/5g dose, followed by images 0 and 55msec after the R-wave. Hearts were then harvested, sliced 1mm thick and stained with TTC. All animals survived surgery and imaging; all demonstrated obvious delayed contrast enhancement in the left ventricular wall in Omnipaque images. Fenestra VC revealed cardiac functional changes quantified by low ejection fractions. All subjects demonstrated areas of myocardial infarct in the LAD distribution on both TTC staining and micro-CT imaging. CNT enabled gated cardiac micro-CT imaging demonstrates the ability to consistently identify areas of myocardial infarct in mice, providing a powerful tool for the study of cardiovascular biology. Further work is ongoing to streamline the imaging protocol and perform more quantitative analysis of the images.

AB - We demonstrate the application of our cardiac- and respiratory-gated carbon nanotube (CNT) micro-CT system by evaluating murine myocardial infarction models with a delayed contrast enhancement technique. Myocardial infarction was induced in 8 wild-type male mice. The ischemia reperfusion model was achieved by surgical occlusion of the LAD artery for 30 minutes followed by 24 hours of reperfusion. Free-breathing subjects were anesthetized with isoflurane during imaging. Respiratory and cardiac signals were monitored externally to gate the scan. Micro-CT data was obtained at 50kV, 3mA cathode current for 15ms per projection. All images were acquired during end exhalation at either 0msec or 55msec after the R-wave (diastole or systole, respectively). Following administration of Omnipaque 300mgI/mL at 0.1ml/5g, images were obtained at 0msec after the R-wave. Fenestra VC was then administered at a 0.1ml/5g dose, followed by images 0 and 55msec after the R-wave. Hearts were then harvested, sliced 1mm thick and stained with TTC. All animals survived surgery and imaging; all demonstrated obvious delayed contrast enhancement in the left ventricular wall in Omnipaque images. Fenestra VC revealed cardiac functional changes quantified by low ejection fractions. All subjects demonstrated areas of myocardial infarct in the LAD distribution on both TTC staining and micro-CT imaging. CNT enabled gated cardiac micro-CT imaging demonstrates the ability to consistently identify areas of myocardial infarct in mice, providing a powerful tool for the study of cardiovascular biology. Further work is ongoing to streamline the imaging protocol and perform more quantitative analysis of the images.

KW - Carbon nanotube

KW - Computed tomography

KW - Gated imaging

KW - Micro-CT

KW - Murine model

KW - Myocardial infarct

UR - http://www.scopus.com/inward/record.url?scp=79955828889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955828889&partnerID=8YFLogxK

U2 - 10.1117/12.878168

DO - 10.1117/12.878168

M3 - Conference contribution

AN - SCOPUS:79955828889

SN - 9780819485076

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Medical Imaging 2011

ER -