Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement

Laurel M. Burk, Kohan Wang, Eunice Kang, Mauricio Rojas, Monte Willis, Yueh Z. Lee, Jianping Lu, Otto Zhou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We demonstrate the application of our cardiac- and respiratory-gated carbon nanotube (CNT) micro-CT system by evaluating murine myocardial infarction models with a delayed contrast enhancement technique. Myocardial infarction was induced in 8 wild-type male mice. The ischemia reperfusion model was achieved by surgical occlusion of the LAD artery for 30 minutes followed by 24 hours of reperfusion. Free-breathing subjects were anesthetized with isoflurane during imaging. Respiratory and cardiac signals were monitored externally to gate the scan. Micro-CT data was obtained at 50kV, 3mA cathode current for 15ms per projection. All images were acquired during end exhalation at either 0msec or 55msec after the R-wave (diastole or systole, respectively). Following administration of Omnipaque 300mgI/mL at 0.1ml/5g, images were obtained at 0msec after the R-wave. Fenestra VC was then administered at a 0.1ml/5g dose, followed by images 0 and 55msec after the R-wave. Hearts were then harvested, sliced 1mm thick and stained with TTC. All animals survived surgery and imaging; all demonstrated obvious delayed contrast enhancement in the left ventricular wall in Omnipaque images. Fenestra VC revealed cardiac functional changes quantified by low ejection fractions. All subjects demonstrated areas of myocardial infarct in the LAD distribution on both TTC staining and micro-CT imaging. CNT enabled gated cardiac micro-CT imaging demonstrates the ability to consistently identify areas of myocardial infarct in mice, providing a powerful tool for the study of cardiovascular biology. Further work is ongoing to streamline the imaging protocol and perform more quantitative analysis of the images.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2011
Subtitle of host publicationBiomedical Applications in Molecular, Structural, and Functional Imaging
DOIs
StatePublished - May 16 2011
EventMedical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging - Lake Buena Vista, FL, United States
Duration: Feb 13 2011Feb 16 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7965
ISSN (Print)1605-7422

Conference

ConferenceMedical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging
CountryUnited States
CityLake Buena Vista, FL
Period2/13/112/16/11

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Keywords

  • Carbon nanotube
  • Computed tomography
  • Gated imaging
  • Micro-CT
  • Murine model
  • Myocardial infarct

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Burk, L. M., Wang, K., Kang, E., Rojas, M., Willis, M., Lee, Y. Z., Lu, J., & Zhou, O. (2011). Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement. In Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging [79651N] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7965). https://doi.org/10.1117/12.878168