Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice

Josef Vormoor, Tsvee Lapidot, Françoise Pflumio, Grant Risdon, Bruce Patterson, Hal Broxmeyer, John E. Dick

Research output: Contribution to journalArticle

231 Citations (Scopus)

Abstract

Unseparated or Ficoll-Hypaque (Pharmacia, Piscataway, NJ)-fractionated human cord blood cells were transplanted into sublethally irradiated severe combined immunodeficient (SCID) mice. High levels of multilineage engraftment, including myeloid and lymphoid lineages, were obtained with 80% of the donor samples as assessed by DNA analysis, fluorescence-activated cell sorting (FACS), and morphology. In contrast to previous and concurrent studies with adult human bone marrow (BM), treatment with human cytokines was not required to establish high-level human cell engraftment, suggesting that neonatal cells either respond differently to the murine microenvironment or they provide their own cytokines in a paracrine fashion. Committed and multipotential myelo-erythroid progenitors were detected using in vitro colony assays and FACS analysis of the murine BM showed the presence of immature CD34+ cells. In addition, human hematopoiesis was maintained for at least 14 weeks providing further evidence that immature hematopoietic precursors had engrafted the murine BM. This in vivo model for human cord blood-derived hematopoiesis will be useful to gain new insights into the biology of neonatal hematopoietic cells and to evaluate their role in gene therapy. There is growing evidence that there are ontogeny-related changes in immature human hematopoietic cells, and therefore, the animal models we have developed for adult and neonatal human hematopoiesis provide useful tools to evaluate these changes in vivo.

Original languageEnglish (US)
Pages (from-to)2489-2497
Number of pages9
JournalBlood
Volume83
Issue number9
StatePublished - May 1 1994
Externally publishedYes

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SCID Mice
Fetal Blood
Bone
Blood
Cells
Sorting
Fluorescence
Cytokines
Diatrizoate
Gene therapy
Ficoll
Hematopoiesis
Bone Marrow
Assays
Animals
Flow Cytometry
DNA
Genetic Therapy
Blood Cells
Cohort Studies

ASJC Scopus subject areas

  • Hematology

Cite this

Vormoor, J., Lapidot, T., Pflumio, F., Risdon, G., Patterson, B., Broxmeyer, H., & Dick, J. E. (1994). Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. Blood, 83(9), 2489-2497.

Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. / Vormoor, Josef; Lapidot, Tsvee; Pflumio, Françoise; Risdon, Grant; Patterson, Bruce; Broxmeyer, Hal; Dick, John E.

In: Blood, Vol. 83, No. 9, 01.05.1994, p. 2489-2497.

Research output: Contribution to journalArticle

Vormoor, J, Lapidot, T, Pflumio, F, Risdon, G, Patterson, B, Broxmeyer, H & Dick, JE 1994, 'Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice', Blood, vol. 83, no. 9, pp. 2489-2497.
Vormoor J, Lapidot T, Pflumio F, Risdon G, Patterson B, Broxmeyer H et al. Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. Blood. 1994 May 1;83(9):2489-2497.
Vormoor, Josef ; Lapidot, Tsvee ; Pflumio, Françoise ; Risdon, Grant ; Patterson, Bruce ; Broxmeyer, Hal ; Dick, John E. / Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. In: Blood. 1994 ; Vol. 83, No. 9. pp. 2489-2497.
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