Because generalized anaphylaxis frequently takes place after the intravenous (IV) injection of antigen with the dye 72 hours after the intradermal (ID) injection of the PCA positive antiserum, a 20 fold dilution of the antiserum was carried out in a study of long term skin sensitization. The latter and the PCA reactivities could be distinguished by various methods, namely, 2 mercaptoethanol treatment, heat inactivation, different initiations during the hyperimmunization course, and the relatively selective distribution among the fractions from a DEAE cellulose column. The relative amount of antibody activity absorbed by a rabbit anti guineapig IgG antiserum also showed some difference between the above mentioned reactivities. Presumably the PCA and the longterm skin sensitization activities are not necessarily associated with the same kind of antibody.
|Original language||English (US)|
|Number of pages||7|
|State||Published - Dec 1 1975|
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