Immune response during hyperimmunization

(XII) Long term skin binding antibody in comparison with the ordinary passive cutaneous anaphylaxis (PCA) antibody in immune sera

S. Y. Chiu, J. H. Gonq, Chao-Hung Lee

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Because generalized anaphylaxis frequently takes place after the intravenous (IV) injection of antigen with the dye 72 hours after the intradermal (ID) injection of the PCA positive antiserum, a 20 fold dilution of the antiserum was carried out in a study of long term skin sensitization. The latter and the PCA reactivities could be distinguished by various methods, namely, 2 mercaptoethanol treatment, heat inactivation, different initiations during the hyperimmunization course, and the relatively selective distribution among the fractions from a DEAE cellulose column. The relative amount of antibody activity absorbed by a rabbit anti guineapig IgG antiserum also showed some difference between the above mentioned reactivities. Presumably the PCA and the longterm skin sensitization activities are not necessarily associated with the same kind of antibody.

Original languageEnglish (US)
Title of host publicationJ.FORMOSAN MED.ASS.
Pages613-619
Number of pages7
Volume74
Edition9
StatePublished - 1975
Externally publishedYes

Fingerprint

Passive Cutaneous Anaphylaxis
Immune Sera
Skin
Antibodies
Intradermal Injections
DEAE-Cellulose
Mercaptoethanol
Anaphylaxis
Intravenous Injections
Coloring Agents
Hot Temperature
Rabbits
Antigens
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Immune response during hyperimmunization : (XII) Long term skin binding antibody in comparison with the ordinary passive cutaneous anaphylaxis (PCA) antibody in immune sera. / Chiu, S. Y.; Gonq, J. H.; Lee, Chao-Hung.

J.FORMOSAN MED.ASS.. Vol. 74 9. ed. 1975. p. 613-619.

Research output: Chapter in Book/Report/Conference proceedingChapter

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