Immunization of rabbits against a bacterial pathogen with an alginate microparticle vaccine

Mark A. Suckow, Lamis Z. Jarvinen, Harm HogenEsch, Kinam Park, Terry L. Bowersock

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Pasteurella multocida is an important bacterial pathogen of domestic rabbits. To evaluate the ability of a thiocyanate extract (PTE) of P. multocida to stimulate an immune response and protect against infection with P. multocida, rabbits were immunized subcutaneously or intranasally on Days 7, 21 and 35. Cholera toxin, a potent mucosal adjuvant, was included in one treatment group. Rabbits immunized subcutaneously (SC) or intranasally (IN) had significant increases in serum anti-PTE IgG but not IgA. In contrast, only rabbits immunized IN with PTE developed significant titers of nasal lavage anti-PTE IgA and cholera toxin significantly enhanced this response. In a second study rabbits were immunized via the drinking water with PTE incorporated into alginate microparticles on Days 7, 14 and 21. Mild increases in serum IgG were noted in rabbits immunized with PTE in microparticles, with or without cholera toxin, and this increase was significant (P≤0.05) on Day 21 for rabbits receiving PTE and cholera toxin. Nasal lavage anti-PTE IgA was significantly (P≤0.05) increased in rabbits immunized orally with PTE, with or without cholera toxin, in microparticles. This effect was not enhanced by cholera toxin. Rabbits orally immunized with PTE in microparticles had significantly fewer colony forming units of homologous P. multocida recovered from the lungs and nasopharynx following an intranasal challenge. These results demonstrate that PTE incorporated into alginate microparticles and administered orally is immunogenic and confers protective immunity.

Original languageEnglish (US)
Pages (from-to)227-235
Number of pages9
JournalJournal of Controlled Release
Volume85
Issue number1-3
DOIs
StatePublished - Dec 13 2002
Externally publishedYes

Fingerprint

Immunization
Vaccines
Cholera Toxin
Rabbits
Pasteurella multocida
Nasal Lavage
alginic acid
Nasopharynx
Serum
Drinking Water
Immunoglobulin A
Immunity
Stem Cells
Immunoglobulin G
Lung
Infection

Keywords

  • Alginate
  • Microparticles
  • Pasteurella multocida
  • Rabbit

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Immunization of rabbits against a bacterial pathogen with an alginate microparticle vaccine. / Suckow, Mark A.; Jarvinen, Lamis Z.; HogenEsch, Harm; Park, Kinam; Bowersock, Terry L.

In: Journal of Controlled Release, Vol. 85, No. 1-3, 13.12.2002, p. 227-235.

Research output: Contribution to journalArticle

Suckow, Mark A. ; Jarvinen, Lamis Z. ; HogenEsch, Harm ; Park, Kinam ; Bowersock, Terry L. / Immunization of rabbits against a bacterial pathogen with an alginate microparticle vaccine. In: Journal of Controlled Release. 2002 ; Vol. 85, No. 1-3. pp. 227-235.
@article{21d0c5280a4948af951dd2b7ebff2048,
title = "Immunization of rabbits against a bacterial pathogen with an alginate microparticle vaccine",
abstract = "Pasteurella multocida is an important bacterial pathogen of domestic rabbits. To evaluate the ability of a thiocyanate extract (PTE) of P. multocida to stimulate an immune response and protect against infection with P. multocida, rabbits were immunized subcutaneously or intranasally on Days 7, 21 and 35. Cholera toxin, a potent mucosal adjuvant, was included in one treatment group. Rabbits immunized subcutaneously (SC) or intranasally (IN) had significant increases in serum anti-PTE IgG but not IgA. In contrast, only rabbits immunized IN with PTE developed significant titers of nasal lavage anti-PTE IgA and cholera toxin significantly enhanced this response. In a second study rabbits were immunized via the drinking water with PTE incorporated into alginate microparticles on Days 7, 14 and 21. Mild increases in serum IgG were noted in rabbits immunized with PTE in microparticles, with or without cholera toxin, and this increase was significant (P≤0.05) on Day 21 for rabbits receiving PTE and cholera toxin. Nasal lavage anti-PTE IgA was significantly (P≤0.05) increased in rabbits immunized orally with PTE, with or without cholera toxin, in microparticles. This effect was not enhanced by cholera toxin. Rabbits orally immunized with PTE in microparticles had significantly fewer colony forming units of homologous P. multocida recovered from the lungs and nasopharynx following an intranasal challenge. These results demonstrate that PTE incorporated into alginate microparticles and administered orally is immunogenic and confers protective immunity.",
keywords = "Alginate, Microparticles, Pasteurella multocida, Rabbit",
author = "Suckow, {Mark A.} and Jarvinen, {Lamis Z.} and Harm HogenEsch and Kinam Park and Bowersock, {Terry L.}",
year = "2002",
month = "12",
day = "13",
doi = "10.1016/S0168-3659(02)00277-8",
language = "English (US)",
volume = "85",
pages = "227--235",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Immunization of rabbits against a bacterial pathogen with an alginate microparticle vaccine

AU - Suckow, Mark A.

AU - Jarvinen, Lamis Z.

AU - HogenEsch, Harm

AU - Park, Kinam

AU - Bowersock, Terry L.

PY - 2002/12/13

Y1 - 2002/12/13

N2 - Pasteurella multocida is an important bacterial pathogen of domestic rabbits. To evaluate the ability of a thiocyanate extract (PTE) of P. multocida to stimulate an immune response and protect against infection with P. multocida, rabbits were immunized subcutaneously or intranasally on Days 7, 21 and 35. Cholera toxin, a potent mucosal adjuvant, was included in one treatment group. Rabbits immunized subcutaneously (SC) or intranasally (IN) had significant increases in serum anti-PTE IgG but not IgA. In contrast, only rabbits immunized IN with PTE developed significant titers of nasal lavage anti-PTE IgA and cholera toxin significantly enhanced this response. In a second study rabbits were immunized via the drinking water with PTE incorporated into alginate microparticles on Days 7, 14 and 21. Mild increases in serum IgG were noted in rabbits immunized with PTE in microparticles, with or without cholera toxin, and this increase was significant (P≤0.05) on Day 21 for rabbits receiving PTE and cholera toxin. Nasal lavage anti-PTE IgA was significantly (P≤0.05) increased in rabbits immunized orally with PTE, with or without cholera toxin, in microparticles. This effect was not enhanced by cholera toxin. Rabbits orally immunized with PTE in microparticles had significantly fewer colony forming units of homologous P. multocida recovered from the lungs and nasopharynx following an intranasal challenge. These results demonstrate that PTE incorporated into alginate microparticles and administered orally is immunogenic and confers protective immunity.

AB - Pasteurella multocida is an important bacterial pathogen of domestic rabbits. To evaluate the ability of a thiocyanate extract (PTE) of P. multocida to stimulate an immune response and protect against infection with P. multocida, rabbits were immunized subcutaneously or intranasally on Days 7, 21 and 35. Cholera toxin, a potent mucosal adjuvant, was included in one treatment group. Rabbits immunized subcutaneously (SC) or intranasally (IN) had significant increases in serum anti-PTE IgG but not IgA. In contrast, only rabbits immunized IN with PTE developed significant titers of nasal lavage anti-PTE IgA and cholera toxin significantly enhanced this response. In a second study rabbits were immunized via the drinking water with PTE incorporated into alginate microparticles on Days 7, 14 and 21. Mild increases in serum IgG were noted in rabbits immunized with PTE in microparticles, with or without cholera toxin, and this increase was significant (P≤0.05) on Day 21 for rabbits receiving PTE and cholera toxin. Nasal lavage anti-PTE IgA was significantly (P≤0.05) increased in rabbits immunized orally with PTE, with or without cholera toxin, in microparticles. This effect was not enhanced by cholera toxin. Rabbits orally immunized with PTE in microparticles had significantly fewer colony forming units of homologous P. multocida recovered from the lungs and nasopharynx following an intranasal challenge. These results demonstrate that PTE incorporated into alginate microparticles and administered orally is immunogenic and confers protective immunity.

KW - Alginate

KW - Microparticles

KW - Pasteurella multocida

KW - Rabbit

UR - http://www.scopus.com/inward/record.url?scp=0037073644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037073644&partnerID=8YFLogxK

U2 - 10.1016/S0168-3659(02)00277-8

DO - 10.1016/S0168-3659(02)00277-8

M3 - Article

C2 - 12480327

AN - SCOPUS:0037073644

VL - 85

SP - 227

EP - 235

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 1-3

ER -