Immunization with DNA, adenovirus or both in biodegradable alginate microspheres: Effect of route of inoculation on immune response

Suresh K. Mittal, Neeraj Aggarwal, G. Sailaja, Alberto Van Olphen, Harm Hogenesch, Adam North, John Hays, Stanley Moffatt

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

To determine the potential for biodegradable alginate microspheres to be used as a delivery vehicle for DNA based vaccines, we constructed a plasmid, pMNe-gal-SV40, containing the bacterial β-galactosidase (LacZ) gene under the control of the murine cytomegalovirus (MCMV) immediate-early promoter and the simian virus 40 (SV40) polyadenylation signal. The effect of the route of administration and co-administration of adenovirus on systemic and mucosal immune responses were investigated. Mice were inoculated orally, intranasally (i.n.), intramuscularly (i.m.), subcutaneously (s.c.) or intraperitoneally (i.p.) on days 0, 14 and 28 with microspheres containing plasmid DNA, bovine adenovirus type 3 (BAd3) or plasmid DNA + BAd3. Systemic routes of immunization (i.m., s.c. and i.p.) resulted in higher LacZ- or BAd3-specific IgG ELISA titers compared to those obtained by mucosal routes of inoculation (oral and i.n.). Mucosal immunization led to slightly higher titers of LacZ- or BAd3-specific IgA at mucosal sites compared to those obtained by the various systemic routes. All the routes of immunization induced LacZ-specific lymphoproliferation. Co-administration of BAd3 enhanced the LacZ-specific IgG response irrespective of the route of administration. (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalVaccine
Volume19
Issue number2-3
DOIs
StatePublished - Sep 15 2000

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Keywords

  • Adenovirus
  • Biodegradable microsphere
  • DNA immunization
  • Gene delivery vehicle
  • Mucosal immune response
  • Systemic immune response

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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