Immunocytochemical study of retinal diode laser photocoagulation in the rat

P. R.S. Richardson, M. E. Boulton, J. Duvall-Young, D. McLeod

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Abstract

Aim - To determine the nature of the cellular infiltrate, alterations in cell adhesion molecules, and MHC II antigen expression in the rat retina following diode laser retinal photocoagulation. Method - 20 normal Lister rats underwent diode laser photocoagulation of the retina. Frozen sections from eyes enucleated at 0, 1, 5, 13, and 33 days post laser were examined for T cells (R7.3), CD4 T cells (W3/25), activated CD4 T cells (OX-40), CD8 T cells (OX-8), B cells (OX-33), and macrophages (OX-42), MHC II antigen (OX-6), and E-Selectin-1, VCAM-1, and ICAM-1. Results - Retinal diode laser photocoagulation stimulated a wound healing response in the outer retina and choroid. The cellular infiltrate included macrophages and activated CD4 T cells at 13 and 33 days post laser. Glial cells in the inner plexiform and inner nuclear layers expressed MHC II antigen at 24 hours only. ICAM-1 antigen was induced in RPE cells and in Muller cells in the inner retina at all time intervals post laser and intense staining for ICAM-1 was present around intraretinal migrated cells at 13 and 33 days post laser. VCAM-1 antigen expression was induced in the choroidal vascular endothelium and RPE at 13 and 33 days after laser as was E-Selectin-1 antigen expression which was also evident focally sat the external limiting membrane in association with migrated cells adjacent to the burn. Conclusions - These results suggest that alterations in cell adhesion molecules may regulate the migration and activation of retinal pigment epithelium, macrophages sand CD4 T cells at the outer blood-retinal barrier and choroid following diode laser photocoagulation of the normal Lister rat retina.

Original languageEnglish (US)
Pages (from-to)1092-1098
Number of pages7
JournalBritish Journal of Ophthalmology
Volume80
Issue number12
DOIs
StatePublished - Dec 1996

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Richardson, P. R. S., Boulton, M. E., Duvall-Young, J., & McLeod, D. (1996). Immunocytochemical study of retinal diode laser photocoagulation in the rat. British Journal of Ophthalmology, 80(12), 1092-1098. https://doi.org/10.1136/bjo.80.12.1092