Immunocytochemistry performed on the cell-transferred direct smears of the fine-needle aspirates

Howard Wu, Kelly J. Jones, Harvey Cramer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Immunocytochemistry (ICC) performed on the cell-transferred cytologic smears (CTCS) of fine-needle aspiration (FNA) is useful when the cell blocks lack adequate material. The comparison of the ICC results from the CTCS of FNA with the corresponding formalin-fixed paraffin-embedded tissue (FFPE) has not been reported previously. We applied 12 commonly used ICC antibodies on 160 pieces of ethanol-fixed, cell-transferred Papanicolaou-stained smears obtained from 42 FNA specimens and compared the staining results with the corresponding FFPE on which the same panel of immunostains was performed. Of the 160 pieces of transferred materials, only 3 (1.9%) were lost during specimen processing. In total, 153 of 157 (97.5%) showed staining results that agreed with the corresponding FFPE, including 78 of 81 positive staining and 75 of 76 negative staining cases. ICC performed on the cell-transferred FNA smears is reliable and shows staining results highly comparable with the corresponding FFPE tissue.

Original languageEnglish
Pages (from-to)754-758
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume139
Issue number6
DOIs
StatePublished - Jun 2013

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Needles
Fine Needle Biopsy
Paraffin
Immunohistochemistry
Formaldehyde
Staining and Labeling
Papanicolaou Test
Negative Staining
Ethanol
Antibodies

Keywords

  • Cell-transfer
  • Cytology
  • Fine-needle aspiration
  • Immunocytochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunocytochemistry performed on the cell-transferred direct smears of the fine-needle aspirates. / Wu, Howard; Jones, Kelly J.; Cramer, Harvey.

In: American Journal of Clinical Pathology, Vol. 139, No. 6, 06.2013, p. 754-758.

Research output: Contribution to journalArticle

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