Immunodominant antigens of Streptococcus mutans in dental caries-resistant subjects

Richard Gregory, L. C. Hobbs, J. C. Kindle, T. VanTo, H. S. Malmstrom

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Previously, we reported that dental caries-resistant subjects, who have significantly fewer streptococcus mutans in whole saliva than caries-susceptible patients, have significantly higher levels of naturally occurring binding and neutralizing parotid salivary immunoglobulin A and serum immunoglobulin G antibodies to native S. mutans antigens than caries-susceptible patients. Recent animal studies indicated that the immunogenicity of swallowed S. mutans may be altered by either saliva-coating or stomach acid-denaturation. These results suggest a difference not only in the quantity of antibody to S. mutans, but also in the antigenic epitopes that caries-resistant subjects synthesize antibody to as compared with caries-susceptible patients. In the present report, sodium dodecyl sulfate-polyacrylamide gel electrophoresis/immunoblotting studies indicate that caries-resistant subjects produce salivary immunoglobulin A and serum immunoglobulin G antibodies to several different (molecular weight: 94, 80, 40, and 35 kilodaltons) as well as several similar (molecular weight: 67, 55, and 30 kilodaltons) S. mutans epitopes as compared to caries-susceptible patients. This provides additional confirmation for our previous binding and functional antibody studies, indicating that caries-resistant subjects synthesize antibodies of different specificities than caries-susceptible patients. This study supports the concept of immune regulation of dental caries by naturally occurring antibodies induced by swallowing S. mutans antigens in saliva.

Original languageEnglish (US)
Pages (from-to)132-136
Number of pages5
JournalHuman Antibodies and Hybridomas
Volume1
Issue number3
StatePublished - 1990
Externally publishedYes

Fingerprint

Immunodominant Epitopes
Streptococcus mutans
Dental Caries
Antibodies
Saliva
Immunoglobulin A
Epitopes
Immunoglobulin G
Molecular Weight
Antigens
Antibody Specificity
Deglutition
Serum
Immunoblotting
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Stomach
Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Gregory, R., Hobbs, L. C., Kindle, J. C., VanTo, T., & Malmstrom, H. S. (1990). Immunodominant antigens of Streptococcus mutans in dental caries-resistant subjects. Human Antibodies and Hybridomas, 1(3), 132-136.

Immunodominant antigens of Streptococcus mutans in dental caries-resistant subjects. / Gregory, Richard; Hobbs, L. C.; Kindle, J. C.; VanTo, T.; Malmstrom, H. S.

In: Human Antibodies and Hybridomas, Vol. 1, No. 3, 1990, p. 132-136.

Research output: Contribution to journalArticle

Gregory, R, Hobbs, LC, Kindle, JC, VanTo, T & Malmstrom, HS 1990, 'Immunodominant antigens of Streptococcus mutans in dental caries-resistant subjects', Human Antibodies and Hybridomas, vol. 1, no. 3, pp. 132-136.
Gregory, Richard ; Hobbs, L. C. ; Kindle, J. C. ; VanTo, T. ; Malmstrom, H. S. / Immunodominant antigens of Streptococcus mutans in dental caries-resistant subjects. In: Human Antibodies and Hybridomas. 1990 ; Vol. 1, No. 3. pp. 132-136.
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