Immunogenicity of a multi-epitope DNA vaccine against hantavirus

Chen Zhao, Ying Sun, Yujie Zhao, Si Wang, Tongtong Yu, Feng Du, X. Frank Yang, Enjie Luo

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV.

Original languageEnglish (US)
Pages (from-to)208-215
Number of pages8
JournalHuman Vaccines and Immunotherapeutics
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2012

Keywords

  • Chemical gene synthesis
  • Glycoprotein
  • Hantavirus
  • HTNV
  • Multi-epitope DNA vaccine
  • PUUV
  • SEOV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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