Immunogenicity of a multi-epitope DNA vaccine against hantavirus

Chen Zhao, Ying Sun, Yujie Zhao, Si Wang, Tongtong Yu, Feng Du, X. Frank Yang, Enjie Luo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV.

Original languageEnglish (US)
Pages (from-to)208-215
Number of pages8
JournalHuman Vaccines and Immunotherapeutics
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

Hantavirus
Seoul virus
DNA Vaccines
Hantaan virus
Epitopes
Hemorrhagic Fever with Renal Syndrome
Puumala virus
Infection
China
Glycoproteins
Vaccination
Viruses
Mutation
Genes

Keywords

  • Chemical gene synthesis
  • Glycoprotein
  • Hantavirus
  • HTNV
  • Multi-epitope DNA vaccine
  • PUUV
  • SEOV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Zhao, C., Sun, Y., Zhao, Y., Wang, S., Yu, T., Du, F., ... Luo, E. (2012). Immunogenicity of a multi-epitope DNA vaccine against hantavirus. Human Vaccines and Immunotherapeutics, 8(2), 208-215. https://doi.org/10.4161/hv.18389

Immunogenicity of a multi-epitope DNA vaccine against hantavirus. / Zhao, Chen; Sun, Ying; Zhao, Yujie; Wang, Si; Yu, Tongtong; Du, Feng; Yang, X. Frank; Luo, Enjie.

In: Human Vaccines and Immunotherapeutics, Vol. 8, No. 2, 01.02.2012, p. 208-215.

Research output: Contribution to journalArticle

Zhao, C, Sun, Y, Zhao, Y, Wang, S, Yu, T, Du, F, Yang, XF & Luo, E 2012, 'Immunogenicity of a multi-epitope DNA vaccine against hantavirus', Human Vaccines and Immunotherapeutics, vol. 8, no. 2, pp. 208-215. https://doi.org/10.4161/hv.18389
Zhao, Chen ; Sun, Ying ; Zhao, Yujie ; Wang, Si ; Yu, Tongtong ; Du, Feng ; Yang, X. Frank ; Luo, Enjie. / Immunogenicity of a multi-epitope DNA vaccine against hantavirus. In: Human Vaccines and Immunotherapeutics. 2012 ; Vol. 8, No. 2. pp. 208-215.
@article{778747574b1d45d0b4be229989fce54b,
title = "Immunogenicity of a multi-epitope DNA vaccine against hantavirus",
abstract = "Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV.",
keywords = "Chemical gene synthesis, Glycoprotein, Hantavirus, HTNV, Multi-epitope DNA vaccine, PUUV, SEOV",
author = "Chen Zhao and Ying Sun and Yujie Zhao and Si Wang and Tongtong Yu and Feng Du and Yang, {X. Frank} and Enjie Luo",
year = "2012",
month = "2",
day = "1",
doi = "10.4161/hv.18389",
language = "English (US)",
volume = "8",
pages = "208--215",
journal = "Human Vaccines and Immunotherapeutics",
issn = "2164-5515",
publisher = "Landes Bioscience",
number = "2",

}

TY - JOUR

T1 - Immunogenicity of a multi-epitope DNA vaccine against hantavirus

AU - Zhao, Chen

AU - Sun, Ying

AU - Zhao, Yujie

AU - Wang, Si

AU - Yu, Tongtong

AU - Du, Feng

AU - Yang, X. Frank

AU - Luo, Enjie

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV.

AB - Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV.

KW - Chemical gene synthesis

KW - Glycoprotein

KW - Hantavirus

KW - HTNV

KW - Multi-epitope DNA vaccine

KW - PUUV

KW - SEOV

UR - http://www.scopus.com/inward/record.url?scp=84861831203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861831203&partnerID=8YFLogxK

U2 - 10.4161/hv.18389

DO - 10.4161/hv.18389

M3 - Article

C2 - 22426376

AN - SCOPUS:84861831203

VL - 8

SP - 208

EP - 215

JO - Human Vaccines and Immunotherapeutics

JF - Human Vaccines and Immunotherapeutics

SN - 2164-5515

IS - 2

ER -