Immunohistochemical detection and localization of somatostatin receptor subtypes in prostate tissue from patients with bladder outlet obstruction

Rodolfo Montironi, Liang Cheng, Roberta Mazzucchelli, Doriana Morichetti, Daniela Stramazzotti, Alfredo Santinelli, Gianluca Moroncini, Andrea B. Galosi, Giovanni Muzzonigro, Giancarlo Comeri, Jon Lovisolo, Sergio Cosciani-Cunico, Aldo V. Bono

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and aim of the study: Scant information on the cellular distribution of the five somatostatin receptor (SSTR) subtypes in the normal prostate and in neoplasms of the prostate has been reported in very few studies in which techniques, such as in situ hybridization histochemistry, autoradiography, and more recently immunohistochemistry, have been applied. The aim of the study was to examine immunohistochemically the distribution and localization of these 5 subtypes in the various tissue components in normal prostate. Materials: The study was conducted in 14 surgical specimens of normal prostate tissue from adenomectomy specimens from patients with bladder outlet obstruction. The distribution and localization of the 5 somatostatin receptor (SSTR) subtypes was investigated with an immunohistochemical technique. Specificity of the antibodies against the 5 receptor subtypes was preliminarily investigated. Results: Close to 90% of secretory cells showed a weak positivity in the cytoplasm, the proportion ranging from 86.3% (SSTR4) to 89.9% (SSTR5). Strong immunoreactivity was seen in a small proportion of cells, ranging from 0.8% (SSTR3) to 3.2% (SSTR1). For the subtypes 1 and 3 the greatest proportion of basal cells showed a moderate intensity (42.5 and 41.4%, respectively), strong immunoreactivity being observed only in 18.1 and 15.8% of cells, respectively. For the subtypes 2, 4 and 5, the majority of cells showed a weak intensity (72.3, 65.7 and 65.1%, respectively). Subtype 1 showed a strong immunoreactivity in the cytoplasm in 60% of the smooth muscle cells. With subtypes 2, 3 and 4 the greatest proportion of cells showed a weak intensity (63.4, 89.8 and 81.7%, respectively). With the subtype 5 the majority of cells (59.8%) were negative. Subtype 1 showed a strong immunoreactivity in the cytoplasm in 98.6% of the endothelial cells. With subtypes 3 and 4 the greatest proportion of cells showed a weak intensity (73.5 and 56.4%, respectively). With the subtype 2 and 5 the majority of cells were negative (59.1 and 50.7%, respectively). Conclusions: Our immunohistochemical study on the SSTRs expands our knowledge in the distribution of these subtypes in the various tissue components in the prostate. Such an information may prove useful in developing further non-surgical strategies for the prevention and treatment of benign prostatic hyperplasia and, in particular, of preneoplastic and neoplastic lesions of the prostate.

Original languageEnglish
Pages (from-to)473-482
Number of pages10
JournalCellular Oncology
Volume30
Issue number6
DOIs
StatePublished - 2008

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Urinary Bladder Neck Obstruction
Somatostatin Receptors
Prostate
Cytoplasm
Antibody Specificity
Prostatic Hyperplasia
Autoradiography
Smooth Muscle Myocytes
In Situ Hybridization
Prostatic Neoplasms
Endothelial Cells
Immunohistochemistry

Keywords

  • Benign prostatic hyperplasia
  • Normal prostate tissue
  • Somatostatin receptors

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine

Cite this

Immunohistochemical detection and localization of somatostatin receptor subtypes in prostate tissue from patients with bladder outlet obstruction. / Montironi, Rodolfo; Cheng, Liang; Mazzucchelli, Roberta; Morichetti, Doriana; Stramazzotti, Daniela; Santinelli, Alfredo; Moroncini, Gianluca; Galosi, Andrea B.; Muzzonigro, Giovanni; Comeri, Giancarlo; Lovisolo, Jon; Cosciani-Cunico, Sergio; Bono, Aldo V.

In: Cellular Oncology, Vol. 30, No. 6, 2008, p. 473-482.

Research output: Contribution to journalArticle

Montironi, R, Cheng, L, Mazzucchelli, R, Morichetti, D, Stramazzotti, D, Santinelli, A, Moroncini, G, Galosi, AB, Muzzonigro, G, Comeri, G, Lovisolo, J, Cosciani-Cunico, S & Bono, AV 2008, 'Immunohistochemical detection and localization of somatostatin receptor subtypes in prostate tissue from patients with bladder outlet obstruction', Cellular Oncology, vol. 30, no. 6, pp. 473-482. https://doi.org/10.3233/CLO-2008-0433
Montironi, Rodolfo ; Cheng, Liang ; Mazzucchelli, Roberta ; Morichetti, Doriana ; Stramazzotti, Daniela ; Santinelli, Alfredo ; Moroncini, Gianluca ; Galosi, Andrea B. ; Muzzonigro, Giovanni ; Comeri, Giancarlo ; Lovisolo, Jon ; Cosciani-Cunico, Sergio ; Bono, Aldo V. / Immunohistochemical detection and localization of somatostatin receptor subtypes in prostate tissue from patients with bladder outlet obstruction. In: Cellular Oncology. 2008 ; Vol. 30, No. 6. pp. 473-482.
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T1 - Immunohistochemical detection and localization of somatostatin receptor subtypes in prostate tissue from patients with bladder outlet obstruction

AU - Montironi, Rodolfo

AU - Cheng, Liang

AU - Mazzucchelli, Roberta

AU - Morichetti, Doriana

AU - Stramazzotti, Daniela

AU - Santinelli, Alfredo

AU - Moroncini, Gianluca

AU - Galosi, Andrea B.

AU - Muzzonigro, Giovanni

AU - Comeri, Giancarlo

AU - Lovisolo, Jon

AU - Cosciani-Cunico, Sergio

AU - Bono, Aldo V.

PY - 2008

Y1 - 2008

N2 - Background and aim of the study: Scant information on the cellular distribution of the five somatostatin receptor (SSTR) subtypes in the normal prostate and in neoplasms of the prostate has been reported in very few studies in which techniques, such as in situ hybridization histochemistry, autoradiography, and more recently immunohistochemistry, have been applied. The aim of the study was to examine immunohistochemically the distribution and localization of these 5 subtypes in the various tissue components in normal prostate. Materials: The study was conducted in 14 surgical specimens of normal prostate tissue from adenomectomy specimens from patients with bladder outlet obstruction. The distribution and localization of the 5 somatostatin receptor (SSTR) subtypes was investigated with an immunohistochemical technique. Specificity of the antibodies against the 5 receptor subtypes was preliminarily investigated. Results: Close to 90% of secretory cells showed a weak positivity in the cytoplasm, the proportion ranging from 86.3% (SSTR4) to 89.9% (SSTR5). Strong immunoreactivity was seen in a small proportion of cells, ranging from 0.8% (SSTR3) to 3.2% (SSTR1). For the subtypes 1 and 3 the greatest proportion of basal cells showed a moderate intensity (42.5 and 41.4%, respectively), strong immunoreactivity being observed only in 18.1 and 15.8% of cells, respectively. For the subtypes 2, 4 and 5, the majority of cells showed a weak intensity (72.3, 65.7 and 65.1%, respectively). Subtype 1 showed a strong immunoreactivity in the cytoplasm in 60% of the smooth muscle cells. With subtypes 2, 3 and 4 the greatest proportion of cells showed a weak intensity (63.4, 89.8 and 81.7%, respectively). With the subtype 5 the majority of cells (59.8%) were negative. Subtype 1 showed a strong immunoreactivity in the cytoplasm in 98.6% of the endothelial cells. With subtypes 3 and 4 the greatest proportion of cells showed a weak intensity (73.5 and 56.4%, respectively). With the subtype 2 and 5 the majority of cells were negative (59.1 and 50.7%, respectively). Conclusions: Our immunohistochemical study on the SSTRs expands our knowledge in the distribution of these subtypes in the various tissue components in the prostate. Such an information may prove useful in developing further non-surgical strategies for the prevention and treatment of benign prostatic hyperplasia and, in particular, of preneoplastic and neoplastic lesions of the prostate.

AB - Background and aim of the study: Scant information on the cellular distribution of the five somatostatin receptor (SSTR) subtypes in the normal prostate and in neoplasms of the prostate has been reported in very few studies in which techniques, such as in situ hybridization histochemistry, autoradiography, and more recently immunohistochemistry, have been applied. The aim of the study was to examine immunohistochemically the distribution and localization of these 5 subtypes in the various tissue components in normal prostate. Materials: The study was conducted in 14 surgical specimens of normal prostate tissue from adenomectomy specimens from patients with bladder outlet obstruction. The distribution and localization of the 5 somatostatin receptor (SSTR) subtypes was investigated with an immunohistochemical technique. Specificity of the antibodies against the 5 receptor subtypes was preliminarily investigated. Results: Close to 90% of secretory cells showed a weak positivity in the cytoplasm, the proportion ranging from 86.3% (SSTR4) to 89.9% (SSTR5). Strong immunoreactivity was seen in a small proportion of cells, ranging from 0.8% (SSTR3) to 3.2% (SSTR1). For the subtypes 1 and 3 the greatest proportion of basal cells showed a moderate intensity (42.5 and 41.4%, respectively), strong immunoreactivity being observed only in 18.1 and 15.8% of cells, respectively. For the subtypes 2, 4 and 5, the majority of cells showed a weak intensity (72.3, 65.7 and 65.1%, respectively). Subtype 1 showed a strong immunoreactivity in the cytoplasm in 60% of the smooth muscle cells. With subtypes 2, 3 and 4 the greatest proportion of cells showed a weak intensity (63.4, 89.8 and 81.7%, respectively). With the subtype 5 the majority of cells (59.8%) were negative. Subtype 1 showed a strong immunoreactivity in the cytoplasm in 98.6% of the endothelial cells. With subtypes 3 and 4 the greatest proportion of cells showed a weak intensity (73.5 and 56.4%, respectively). With the subtype 2 and 5 the majority of cells were negative (59.1 and 50.7%, respectively). Conclusions: Our immunohistochemical study on the SSTRs expands our knowledge in the distribution of these subtypes in the various tissue components in the prostate. Such an information may prove useful in developing further non-surgical strategies for the prevention and treatment of benign prostatic hyperplasia and, in particular, of preneoplastic and neoplastic lesions of the prostate.

KW - Benign prostatic hyperplasia

KW - Normal prostate tissue

KW - Somatostatin receptors

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