Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour

B. Delahunt, John Eble, D. King, P. B. Bethwaite, J. N. Nacey, A. Thornton

Research output: Contribution to journalArticle

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Abstract

Aims: To investigate the histogenesis of paratesticular adenomatoid tumour by use of immunohistochemical markers for a variety of carcinomas and mesothelioma. Methods and results: Immunohistochemical staining of sections from 12 cases of paratesticular adenomatoid tumour was undertaken using primary antibodies to antigens expressed by benign epithelial cells and carcinoma (cytokeratin AE1/AE3, cytokeratin 3413312, epithelial membrane antigen, MOC-31, BerEP4, CEA, B72.3, LEA.135, Leu M1), stromal and vascular markers (vimentin, CD34, factor VIII), and mesothelioma-associated antigens (thrombomodulin, HBME-1, OC 125) and p53 protein. There was absence of immunohistochemical expression of epithelial/carcinoma markers MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1 and to factor VIII and CD34. All tumours expressed cytokeratin AE1/AE3, epithelial membrane antigen and vimentin, with weak expression of cytokeratin 34βE12 in 25% of tumours. Each tumour showed expression of thrombomodulin, HBME-1 and OC 125 in a membranous distribution, p53 protein expression was not detected. Conclusions: The immunohistochemical profile of paratesticular adenomatoid tumour is strongly supportive of a mesothelial cell origin.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalHistopathology
Volume36
Issue number2
DOIs
StatePublished - 2000

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Adenomatoid Tumor
Keratins
Thrombomodulin
Mucin-1
Mesothelioma
Factor VIII
Vimentin
Carcinoma
Neoplasms
Blood Vessels
Proteins
Epithelial Cells
Staining and Labeling
Antigens
Antibodies

Keywords

  • Adenomatoid tumour
  • Immunohistochemistry
  • Mesothelial cell
  • Mesothelioma
  • Pathogenesis

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour. / Delahunt, B.; Eble, John; King, D.; Bethwaite, P. B.; Nacey, J. N.; Thornton, A.

In: Histopathology, Vol. 36, No. 2, 2000, p. 109-115.

Research output: Contribution to journalArticle

Delahunt, B. ; Eble, John ; King, D. ; Bethwaite, P. B. ; Nacey, J. N. ; Thornton, A. / Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour. In: Histopathology. 2000 ; Vol. 36, No. 2. pp. 109-115.
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AB - Aims: To investigate the histogenesis of paratesticular adenomatoid tumour by use of immunohistochemical markers for a variety of carcinomas and mesothelioma. Methods and results: Immunohistochemical staining of sections from 12 cases of paratesticular adenomatoid tumour was undertaken using primary antibodies to antigens expressed by benign epithelial cells and carcinoma (cytokeratin AE1/AE3, cytokeratin 3413312, epithelial membrane antigen, MOC-31, BerEP4, CEA, B72.3, LEA.135, Leu M1), stromal and vascular markers (vimentin, CD34, factor VIII), and mesothelioma-associated antigens (thrombomodulin, HBME-1, OC 125) and p53 protein. There was absence of immunohistochemical expression of epithelial/carcinoma markers MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1 and to factor VIII and CD34. All tumours expressed cytokeratin AE1/AE3, epithelial membrane antigen and vimentin, with weak expression of cytokeratin 34βE12 in 25% of tumours. Each tumour showed expression of thrombomodulin, HBME-1 and OC 125 in a membranous distribution, p53 protein expression was not detected. Conclusions: The immunohistochemical profile of paratesticular adenomatoid tumour is strongly supportive of a mesothelial cell origin.

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