Immunohistochemical expression of prostate stem cell antigen in cystoprostatectomies with incidental prostate cancer

R. Mazzucchelli, F. Barbisan, A. Santinelli, A. Lopez-Beltran, Liang Cheng, M. Scarpelli, Rodolfo Montironi

Research output: Contribution to journalArticle

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Abstract

High expression of Prostate Stem Cell Antigen (PSCA) has been shown to be associated with adverse prognostic features in clinically-diagnosed prostate cancer. The aim of this study is to analyze PSCA expression in cystoprostatectomies with incidental prostate carcinoma (PCa). PSCA expression was evaluated immunohistochemically in normal-looking epithelium (NEp), high-grade prostatic intraepithelial neoplasia (HGPIN) and pT2a Gleason score 6 acinar adenocarcinoma. The evaluation was carried out on 20 cystoprostatectomies (CyPs) with incidental PCa from men with bladder urothelial carcinoma (UC), and 20 radical prostatectomies (RPs) with hormonally untreated PCa from men with clinically detected PCa. Ki-67 was also investigated. The percentages of PSCA positive cells in HGPIN were significantly higher than in NEp (NEp: CyP, mean 2.92% ± standard deviation 6.26%; RP, 3.5% ± 6.46%. HGPIN: CyP, 13.67% ± 12.78%; RP, 14.67% ± 11.34%) (p<0.001). The proportions of positive cells in PCa were greater than in HGPIN (CyP, 20.25% ± 15.96%; RP, 22.58% ± 13.67%) (p<0.001). For Ki-67 labeling, the proportions of positive nuclei in the CyPs significantly increased from NEp through HGPIN to PCa. A similar trend was seen in the RPs. In the CyPs the percentages of PSCA and Ki67 positive cells were lower than in the RPs, the differences between the CyP and RP compartments being not statistically significant. Our findings suggest that PSCA is a marker associated with neoplastic transformation of prostate cells, both in CyPs and RPs. However, there are no significant differences between CyPs with incidental prostate carcinoma and RPs with clinically diagnosed cancer.

Original languageEnglish
Pages (from-to)755-762
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume22
Issue number3
StatePublished - Jul 2009

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Prostate
Prostatic Neoplasms
Stem Cells
Prostatectomy
Antigens
Prostatic Intraepithelial Neoplasia
Carcinoma
Epithelium
Neoplastic Cell Transformation
Ki-67 Antigen
Neoplasm Grading
Urinary Bladder
Adenocarcinoma

Keywords

  • Cystoprostatectomy incidental cancer
  • High-grade prostatic intraepithelial neoplasia
  • Insignificant cancer
  • Prostate cancer
  • Prostate stem cell antigen
  • Prostatic carcinogenesis
  • Significant cancer

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

Mazzucchelli, R., Barbisan, F., Santinelli, A., Lopez-Beltran, A., Cheng, L., Scarpelli, M., & Montironi, R. (2009). Immunohistochemical expression of prostate stem cell antigen in cystoprostatectomies with incidental prostate cancer. International Journal of Immunopathology and Pharmacology, 22(3), 755-762.

Immunohistochemical expression of prostate stem cell antigen in cystoprostatectomies with incidental prostate cancer. / Mazzucchelli, R.; Barbisan, F.; Santinelli, A.; Lopez-Beltran, A.; Cheng, Liang; Scarpelli, M.; Montironi, Rodolfo.

In: International Journal of Immunopathology and Pharmacology, Vol. 22, No. 3, 07.2009, p. 755-762.

Research output: Contribution to journalArticle

Mazzucchelli, R, Barbisan, F, Santinelli, A, Lopez-Beltran, A, Cheng, L, Scarpelli, M & Montironi, R 2009, 'Immunohistochemical expression of prostate stem cell antigen in cystoprostatectomies with incidental prostate cancer', International Journal of Immunopathology and Pharmacology, vol. 22, no. 3, pp. 755-762.
Mazzucchelli, R. ; Barbisan, F. ; Santinelli, A. ; Lopez-Beltran, A. ; Cheng, Liang ; Scarpelli, M. ; Montironi, Rodolfo. / Immunohistochemical expression of prostate stem cell antigen in cystoprostatectomies with incidental prostate cancer. In: International Journal of Immunopathology and Pharmacology. 2009 ; Vol. 22, No. 3. pp. 755-762.
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abstract = "High expression of Prostate Stem Cell Antigen (PSCA) has been shown to be associated with adverse prognostic features in clinically-diagnosed prostate cancer. The aim of this study is to analyze PSCA expression in cystoprostatectomies with incidental prostate carcinoma (PCa). PSCA expression was evaluated immunohistochemically in normal-looking epithelium (NEp), high-grade prostatic intraepithelial neoplasia (HGPIN) and pT2a Gleason score 6 acinar adenocarcinoma. The evaluation was carried out on 20 cystoprostatectomies (CyPs) with incidental PCa from men with bladder urothelial carcinoma (UC), and 20 radical prostatectomies (RPs) with hormonally untreated PCa from men with clinically detected PCa. Ki-67 was also investigated. The percentages of PSCA positive cells in HGPIN were significantly higher than in NEp (NEp: CyP, mean 2.92{\%} ± standard deviation 6.26{\%}; RP, 3.5{\%} ± 6.46{\%}. HGPIN: CyP, 13.67{\%} ± 12.78{\%}; RP, 14.67{\%} ± 11.34{\%}) (p<0.001). The proportions of positive cells in PCa were greater than in HGPIN (CyP, 20.25{\%} ± 15.96{\%}; RP, 22.58{\%} ± 13.67{\%}) (p<0.001). For Ki-67 labeling, the proportions of positive nuclei in the CyPs significantly increased from NEp through HGPIN to PCa. A similar trend was seen in the RPs. In the CyPs the percentages of PSCA and Ki67 positive cells were lower than in the RPs, the differences between the CyP and RP compartments being not statistically significant. Our findings suggest that PSCA is a marker associated with neoplastic transformation of prostate cells, both in CyPs and RPs. However, there are no significant differences between CyPs with incidental prostate carcinoma and RPs with clinically diagnosed cancer.",
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