Immunohistochemical expression of somatostatin receptor subtypes in prostate tissue from cystoprostatectomies with incidental prostate cancer

Doriana Morichetti, Roberta Mazzucchelli, Daniela Stramazzotti, Alfredo Santinelli, Antonio Lopez-Beltran, Marina Scarpelli, Aldo V. Bono, Liang Cheng, Rodolfo Montironi

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE To examine immunohistochemically the expression of the five somatostatin receptors (SSTRs) in cystoprostatectomies (CyPs) with incidental prostate cancer. MATERIALS AND METHODS The five SSTRs (SSTR1-5) were evaluated in 'normal-looking' epithelium (NEp), high-grade prostatic intraepithelial neoplasia (HGPIN) and pT2a Gleason score 6 adenocarcinoma in 20 CyP specimens with incidental prostate cancer and 20 radical prostatectomy (RP) specimens with clinically detected prostate cancer. RESULTS For cytoplasm expression, the mean percentage of positive secretory cells with strong cytoplasmic staining increased from NEp to HGPIN and prostate cancer, being slightly lower in the CyP than in the RP specimens. Both in the CyP and RP specimens SSTR4 was found in the highest percentage of cells. There was membrane staining in the secretory cells for SSTR3 and SSTR4. There was nuclear staining in the secretory cells for SSTR4 and SSTR5. For SSTR1 and SSTR3 the mean proportions of positive basal cells were higher than for the other three subtypes, and greater in NEp than in HGPIN. There were positive smooth muscle and endothelial cells for all five SSTR subtypes, the highest proportions being SSTR1 and the lowest SSTR5. CONCLUSIONS This immunohistochemical study expands our knowledge of the expression and localization of SSTRs in the various tissue components in the prostate with incidental cancer, compared with clinically detected cancer. Such information might be useful in developing further non-invasive strategies for the prevention and treatment of pre-neoplastic and neoplastic lesions of the prostate.

Original languageEnglish
Pages (from-to)1072-1080
Number of pages9
JournalBJU International
Volume106
Issue number7
DOIs
StatePublished - Oct 2010

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Somatostatin Receptors
Prostate
Prostatic Intraepithelial Neoplasia
Prostatic Neoplasms
Prostatectomy
Epithelium
Staining and Labeling
Neoplasm Grading
Smooth Muscle Myocytes
Neoplasms
Cytoplasm
Adenocarcinoma
Endothelial Cells
Membranes
somatostatin receptor type 1

Keywords

  • cystoprostatectomy
  • high-grade prostatic intraepithelial neoplasia
  • incidental cancer
  • prostate cancer
  • somatostatin receptors

ASJC Scopus subject areas

  • Urology
  • Medicine(all)

Cite this

Morichetti, D., Mazzucchelli, R., Stramazzotti, D., Santinelli, A., Lopez-Beltran, A., Scarpelli, M., ... Montironi, R. (2010). Immunohistochemical expression of somatostatin receptor subtypes in prostate tissue from cystoprostatectomies with incidental prostate cancer. BJU International, 106(7), 1072-1080. https://doi.org/10.1111/j.1464-410X.2010.09238.x

Immunohistochemical expression of somatostatin receptor subtypes in prostate tissue from cystoprostatectomies with incidental prostate cancer. / Morichetti, Doriana; Mazzucchelli, Roberta; Stramazzotti, Daniela; Santinelli, Alfredo; Lopez-Beltran, Antonio; Scarpelli, Marina; Bono, Aldo V.; Cheng, Liang; Montironi, Rodolfo.

In: BJU International, Vol. 106, No. 7, 10.2010, p. 1072-1080.

Research output: Contribution to journalArticle

Morichetti, D, Mazzucchelli, R, Stramazzotti, D, Santinelli, A, Lopez-Beltran, A, Scarpelli, M, Bono, AV, Cheng, L & Montironi, R 2010, 'Immunohistochemical expression of somatostatin receptor subtypes in prostate tissue from cystoprostatectomies with incidental prostate cancer', BJU International, vol. 106, no. 7, pp. 1072-1080. https://doi.org/10.1111/j.1464-410X.2010.09238.x
Morichetti, Doriana ; Mazzucchelli, Roberta ; Stramazzotti, Daniela ; Santinelli, Alfredo ; Lopez-Beltran, Antonio ; Scarpelli, Marina ; Bono, Aldo V. ; Cheng, Liang ; Montironi, Rodolfo. / Immunohistochemical expression of somatostatin receptor subtypes in prostate tissue from cystoprostatectomies with incidental prostate cancer. In: BJU International. 2010 ; Vol. 106, No. 7. pp. 1072-1080.
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abstract = "OBJECTIVE To examine immunohistochemically the expression of the five somatostatin receptors (SSTRs) in cystoprostatectomies (CyPs) with incidental prostate cancer. MATERIALS AND METHODS The five SSTRs (SSTR1-5) were evaluated in 'normal-looking' epithelium (NEp), high-grade prostatic intraepithelial neoplasia (HGPIN) and pT2a Gleason score 6 adenocarcinoma in 20 CyP specimens with incidental prostate cancer and 20 radical prostatectomy (RP) specimens with clinically detected prostate cancer. RESULTS For cytoplasm expression, the mean percentage of positive secretory cells with strong cytoplasmic staining increased from NEp to HGPIN and prostate cancer, being slightly lower in the CyP than in the RP specimens. Both in the CyP and RP specimens SSTR4 was found in the highest percentage of cells. There was membrane staining in the secretory cells for SSTR3 and SSTR4. There was nuclear staining in the secretory cells for SSTR4 and SSTR5. For SSTR1 and SSTR3 the mean proportions of positive basal cells were higher than for the other three subtypes, and greater in NEp than in HGPIN. There were positive smooth muscle and endothelial cells for all five SSTR subtypes, the highest proportions being SSTR1 and the lowest SSTR5. CONCLUSIONS This immunohistochemical study expands our knowledge of the expression and localization of SSTRs in the various tissue components in the prostate with incidental cancer, compared with clinically detected cancer. Such information might be useful in developing further non-invasive strategies for the prevention and treatment of pre-neoplastic and neoplastic lesions of the prostate.",
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AU - Morichetti, Doriana

AU - Mazzucchelli, Roberta

AU - Stramazzotti, Daniela

AU - Santinelli, Alfredo

AU - Lopez-Beltran, Antonio

AU - Scarpelli, Marina

AU - Bono, Aldo V.

AU - Cheng, Liang

AU - Montironi, Rodolfo

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N2 - OBJECTIVE To examine immunohistochemically the expression of the five somatostatin receptors (SSTRs) in cystoprostatectomies (CyPs) with incidental prostate cancer. MATERIALS AND METHODS The five SSTRs (SSTR1-5) were evaluated in 'normal-looking' epithelium (NEp), high-grade prostatic intraepithelial neoplasia (HGPIN) and pT2a Gleason score 6 adenocarcinoma in 20 CyP specimens with incidental prostate cancer and 20 radical prostatectomy (RP) specimens with clinically detected prostate cancer. RESULTS For cytoplasm expression, the mean percentage of positive secretory cells with strong cytoplasmic staining increased from NEp to HGPIN and prostate cancer, being slightly lower in the CyP than in the RP specimens. Both in the CyP and RP specimens SSTR4 was found in the highest percentage of cells. There was membrane staining in the secretory cells for SSTR3 and SSTR4. There was nuclear staining in the secretory cells for SSTR4 and SSTR5. For SSTR1 and SSTR3 the mean proportions of positive basal cells were higher than for the other three subtypes, and greater in NEp than in HGPIN. There were positive smooth muscle and endothelial cells for all five SSTR subtypes, the highest proportions being SSTR1 and the lowest SSTR5. CONCLUSIONS This immunohistochemical study expands our knowledge of the expression and localization of SSTRs in the various tissue components in the prostate with incidental cancer, compared with clinically detected cancer. Such information might be useful in developing further non-invasive strategies for the prevention and treatment of pre-neoplastic and neoplastic lesions of the prostate.

AB - OBJECTIVE To examine immunohistochemically the expression of the five somatostatin receptors (SSTRs) in cystoprostatectomies (CyPs) with incidental prostate cancer. MATERIALS AND METHODS The five SSTRs (SSTR1-5) were evaluated in 'normal-looking' epithelium (NEp), high-grade prostatic intraepithelial neoplasia (HGPIN) and pT2a Gleason score 6 adenocarcinoma in 20 CyP specimens with incidental prostate cancer and 20 radical prostatectomy (RP) specimens with clinically detected prostate cancer. RESULTS For cytoplasm expression, the mean percentage of positive secretory cells with strong cytoplasmic staining increased from NEp to HGPIN and prostate cancer, being slightly lower in the CyP than in the RP specimens. Both in the CyP and RP specimens SSTR4 was found in the highest percentage of cells. There was membrane staining in the secretory cells for SSTR3 and SSTR4. There was nuclear staining in the secretory cells for SSTR4 and SSTR5. For SSTR1 and SSTR3 the mean proportions of positive basal cells were higher than for the other three subtypes, and greater in NEp than in HGPIN. There were positive smooth muscle and endothelial cells for all five SSTR subtypes, the highest proportions being SSTR1 and the lowest SSTR5. CONCLUSIONS This immunohistochemical study expands our knowledge of the expression and localization of SSTRs in the various tissue components in the prostate with incidental cancer, compared with clinically detected cancer. Such information might be useful in developing further non-invasive strategies for the prevention and treatment of pre-neoplastic and neoplastic lesions of the prostate.

KW - cystoprostatectomy

KW - high-grade prostatic intraepithelial neoplasia

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KW - prostate cancer

KW - somatostatin receptors

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