Immunohistochemical localization of glutamate and glutaminase in guinea pig trigeminal premotoneurons

Jack E. Turman, Scott H. Chandler

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Previous electrophysiological experiments in guinea pigs from our laboratory [11,36,37] have suggested that synaptic transmission between last-order interneurons (premotoneurons) and trigeminal motoneurons during reflex activation or cortically induced rhythmical jaw movements is mediated by excitatory amino acids (EAAs). In the present study, we performed a series of double-labeling experiments in guinea pigs to determine the location of neurons which contain glutamate or glutaminase and project to the trigeminal motor nucleus (Mo5). This was accomplished by combining immunohistochemical staining and standard retrograde tract-tracing techniques. Injections of a retrograde tracer, colloidal-gold bound to inactivated WGA-HRP (gWGA-HRP), into the trigeminal motor nucleus labeled a column of neurons originating adjacent to Mo5, including the supratrigeminal nucleus, intertrigeminal nucleus and the mesencephalic nucleus of V. The column extended caudally into the parvocellular reticular formation and adjacent trigeminal sensory nucleus oralis and oralis γ subdivision. In all of these regions, immunoreactivity to glutamate or glutaminase was observed co-localized with gWGA-HRP.

Original languageEnglish (US)
Pages (from-to)49-61
Number of pages13
JournalBrain research
Volume634
Issue number1
DOIs
StatePublished - Jan 14 1994

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Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
Glutaminase
Glutamic Acid
Guinea Pigs
Trigeminal Nuclei
Neurons
Gold Colloid
Excitatory Amino Acids
Reticular Formation
Motor Neurons
Interneurons
Jaw
Synaptic Transmission
Gold
Reflex
Staining and Labeling
Injections
Trigeminal Motor Nucleus

Keywords

  • Brain stem
  • Excitatory amino acid
  • Mastication
  • Reticular formation
  • Trigeminal premotoneuron

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Immunohistochemical localization of glutamate and glutaminase in guinea pig trigeminal premotoneurons. / Turman, Jack E.; Chandler, Scott H.

In: Brain research, Vol. 634, No. 1, 14.01.1994, p. 49-61.

Research output: Contribution to journalArticle

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