Immunohistochemical localization of selected early response genes expressed in trabecular bone of young rats given hPTH 1-34

J. D. Liang, J. M. Hock, George Sandusky, R. F. Santerre, J. E. Onyia

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Intermittent administration of parathyroid hormone (PTH) increases trabecular bone mass in vivo by stimulating bone formation. To further characterize the cellular and molecular mediators of the anabolic response to PTH, we examined the effect of intermittent synthetic hPTH 1-34 on the expression and localization of selected early response genes, c-fos, c-jun, c-myc, and IL-6 protein, in bone tissue by immunohistochemistry. Young male Sprague-Dawley rats, 70-100 g, were injected s.c. with 8 μg/100 g PTH or vehicle control, once daily for 5 days. Femurs were harvested 1 and 24 hours after the fifth injection, then fixed, decalcified, processed for wax embedding, and sections were immunostained. Early response genes, c-fos, c- jun and IL-6, were strongly expressed in osteoblasts, osteocytes, and megakaryocytes in bones 1 hour after PTH, when compared with vehicle-treated controls or sections from rats, 24 hours after PTH injection. Osteoblasts, osteocytes, and megakaryocytes were also positive for c-myc but the differences in stain intensity between control and treated groups were marginal. Also, scattered islands of hematopoietic cells in the marrow stained intensely for IL-6 by 1 hour after PTH, but the stain intensity decreased to control level 24 hours after the last PTH injection. Scattered islands of hematopoietic cells in the bone marrow stained more strongly for c-fos than either c-jun or c-myc, but neither localization nor stain intensity were regulated by PTH at the time points examined. We conclude that during the immediate early phase of the anabolic response, PTH regulates c- fos, c-jun, and IL-6 expression in osteoblasts, osteocytes, megakaryocytes, and selected bone marrow hematopoietic cells in bone.

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalCalcified Tissue International
Volume65
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Teriparatide
Parathyroid Hormone
Genes
Osteocytes
Megakaryocytes
Interleukin-6
Osteoblasts
fos Genes
Coloring Agents
Bone and Bones
Bone Marrow Cells
Injections
Cancellous Bone
Waxes
Interleukin-1
Osteogenesis
Femur
Sprague Dawley Rats
Bone Marrow
Immunohistochemistry

Keywords

  • Bone formation
  • c-fos, c-jun, c-myc, cytokine IL-6
  • Early response genes
  • hPTH 1-34
  • Immunohistochemistry
  • Rats

ASJC Scopus subject areas

  • Endocrinology

Cite this

Immunohistochemical localization of selected early response genes expressed in trabecular bone of young rats given hPTH 1-34. / Liang, J. D.; Hock, J. M.; Sandusky, George; Santerre, R. F.; Onyia, J. E.

In: Calcified Tissue International, Vol. 65, No. 5, 1999, p. 369-373.

Research output: Contribution to journalArticle

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