Immunohistochemical staining for S100 protein in the diagnosis of canine amelanotic melanoma.

George Sandusky, W. W. Carlton, K. A. Wightman

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Abstract

Formalin-fixed, paraffin-embedded tissue samples of canine amelanotic melanomas and normal canine tissues were studied immunohistochemically for the presence of S100 protein. Use of the avidin-biotin complex procedure demonstrated variable amounts of S100 protein in the tumor cell cytoplasm and nuclei in 26 of 31 tumors. S100 protein was not observed in some other common canine skin tumors stained by the avidin-biotin complex technique. These were a mast cell tumor, fibrosarcoma, mammary gland adenocarcinoma, histiocytoma, transmissible venereal tumor, and a thyroid gland adenocarcinoma. Among normal tissues the presence of S100 protein was demonstrated in chondrocytes in the trachea, myoepithelial cells in the breast, melanocytes in the skin, some sweat glands and ducts in the skin, stellate cells in the pituitary, and interdigitating reticulum cells in the lymph node and in Peyer's patches. These results indicate that the avidin-biotin complex procedure for demonstrating S100 protein is a useful diagnostic tool in the diagnosis of canine amelanotic melanoma.

Original languageEnglish
Pages (from-to)577-581
Number of pages5
JournalVeterinary Pathology
Volume22
Issue number6
StatePublished - Nov 1985
Externally publishedYes

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Amelanotic Melanoma
S100 Proteins
melanoma
Canidae
avidin
Avidin
Staining and Labeling
biotin
Biotin
dogs
adenocarcinoma
skin (animal)
proteins
Skin
Veterinary Venereal Tumors
Neoplasms
Adenocarcinoma
Histiocytoma
transmissible venereal tumors
sweat glands

ASJC Scopus subject areas

  • veterinary(all)

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Immunohistochemical staining for S100 protein in the diagnosis of canine amelanotic melanoma. / Sandusky, George; Carlton, W. W.; Wightman, K. A.

In: Veterinary Pathology, Vol. 22, No. 6, 11.1985, p. 577-581.

Research output: Contribution to journalArticle

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