Immunohistochemistry of EGF and bFGF in keratoconus

Guiqiu Zhao, Shaohui Liu, Tao Jiang

Research output: Contribution to journalArticlepeer-review


Objective: To observe the expression of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) in normal cornea and keratoconus and discuss their effects on keratoconus. Methods: Twenty-one keratoconuses and 7 normal corneas were detected. Immunohistochemical SABC method was used to detect the expression of EGF and bFGF in cornea. Results: EGF was expressed in epithelium, endothelium and Bowman layer in normal cornea and keratoconus but the positive expression increased in keratoconus. The bFGF was expressed in epithelium, endothelium and stroma of cornea. The strong positive expression was found in the scar tissue in keratoconus stroma. Conclusion: Increasing EGF accelerates DNA synthesis of epithelial cells and stromal cells in keratoconus to cause tissue healing. High level of bFGF in keratoconus accelerate the proliferation of epithelium, stroma and endothelium.

Original languageEnglish (US)
Pages (from-to)273-275
Number of pages3
JournalChinese Ophthalmic Research
Issue number3
StatePublished - Jun 1 2003
Externally publishedYes


  • Basic fibroblast growth factor
  • Epidermal growth factor
  • Human
  • Immunohistochemistry
  • Keratoconus

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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