Immunolocalization of low-affinity prostaglandin E2 receptors, EP1 and EP2, in adult human epidermis

Raymond Konger, Steven D. Billings, Angela B. Thompson, Akira Morimiya, Jack H. Ladenson, Yvonne Landt, Alice P. Pentland, Sunil Badve

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Four prostaglandin (PG)E2 receptors have been described, termed E-series prostaglandin receptors (EP1-EP4), that can be further subclassified as low-affinity (EP1 and EP2) or high-affinity (EP3 and EP4) receptors. Activation of the low-affinity PGE2 receptors is likely to be important in mediating the actions of the high levels of PGE2 found in various pathologic processes. The pattern of expression of these receptors in epidermis, however, is unknown. We therefore examined the immunolocalization of the EP1 and EP2 receptors in human epidermis. The EP1 and EP 2 receptors demonstrated both plasma membrane and perinuclear or nuclear staining within the basal and spinous layers. Within the granular layer, both receptors were expressed in the cytoplasm with a grainy or granular appearance. The major differences were that the EP2 receptor demonstrated a zone of decreased to absent plasma membrane staining in the superficial spinous layer and only scattered cellular staining within the granular layer. In contrast, the EP1 receptor was prominently expressed throughout the stratum granulosum and the plasma membrane staining pattern was seen throughout the spinous layer. In cultured primary human keratinocytes, we also verified the presence of functional EP1 receptor coupled to intracellular calcium mobilization and EP2 receptor coupled to cAMP production.

Original languageEnglish
Pages (from-to)965-970
Number of pages6
JournalJournal of Investigative Dermatology
Volume124
Issue number5
DOIs
StatePublished - May 2005

Fingerprint

Prostaglandin Receptors
Cell membranes
Dinoprostone
Epidermis
Prostaglandin E Receptors
Staining and Labeling
Cell Membrane
Receptors, Prostaglandin E, EP4 Subtype
Pathologic Processes
Chemical activation
Keratinocytes
Calcium
Cytoplasm

Keywords

  • EP receptor
  • EP receptor
  • Immunolocalization
  • Keratinocytes
  • Prostaglandin E
  • Second messengers

ASJC Scopus subject areas

  • Dermatology

Cite this

Immunolocalization of low-affinity prostaglandin E2 receptors, EP1 and EP2, in adult human epidermis. / Konger, Raymond; Billings, Steven D.; Thompson, Angela B.; Morimiya, Akira; Ladenson, Jack H.; Landt, Yvonne; Pentland, Alice P.; Badve, Sunil.

In: Journal of Investigative Dermatology, Vol. 124, No. 5, 05.2005, p. 965-970.

Research output: Contribution to journalArticle

Konger, Raymond ; Billings, Steven D. ; Thompson, Angela B. ; Morimiya, Akira ; Ladenson, Jack H. ; Landt, Yvonne ; Pentland, Alice P. ; Badve, Sunil. / Immunolocalization of low-affinity prostaglandin E2 receptors, EP1 and EP2, in adult human epidermis. In: Journal of Investigative Dermatology. 2005 ; Vol. 124, No. 5. pp. 965-970.
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