Immunolocalization of the Oligodendrocyte Transcription Factor 1 (Olig1) in Brain Tumors

Biagio Azzarelli, Leticia Miravalle, Ruben Vidal

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Recent in situ hybridization studies showed that mRNA levels of OLIG1 and OLIG2 transcription factors are elevated in oligodendrogliomas. We raised polyclonal antibodies against a synthetic peptide homologous to the human transcription factor Olig1 and studied by immunohistochemistry the expression of Olig1 in 84 brain tumors and in non-neoplastic brain tissues. All oligodendrogliomas, oligoastrocytomas, and dysembryoplastic neuroepithelial tumors showed moderate to strong intranuclear immunoreactivity in cells morphologically identified as oligodendrocytes. In addition, some astrocytomas showed a slight to moderate intranuclear immunoreactivity. None of the other neuroepithelial and non-neuroepithelial tumors showed nuclear immunoreactivity. Double immunostaining of oligodendrogliomas, oligoastrocytomas, and glioblastoma multiforme (GBM) using antibodies against Olig1 and GFAP showed the presence of 3 different cell populations: 1) immunopositive for Olig1 and immunonegative for GFAP, histologically identified as oligodendrocytes; 2) immunopositive only for GFAP, histologically identified as astrocytes; and 3) immunonegative for both antibodies ("null cells"), histologically observed as a population of cells usually with round nuclei and a small amount of cytoplasm. The use of double immunostaining facilitated the distinction among these 3 different tumors. In summary, the use of immunohistochemistry using Olig1 antibodies alone or in combination with anti-GFAP antibody, which can be performed in the routine diagnostic setting, may help in the diagnosis of neuroepithelial tumors.

Original languageEnglish
Pages (from-to)170-179
Number of pages10
JournalJournal of Neuropathology and Experimental Neurology
Volume63
Issue number2
StatePublished - Feb 2004

Fingerprint

Oligodendroglia
Oligodendroglioma
Brain Neoplasms
Transcription Factors
Neuroepithelial Neoplasms
Antibodies
Immunohistochemistry
Null Lymphocytes
Astrocytoma
Glioblastoma
Astrocytes
Population
In Situ Hybridization
Anti-Idiotypic Antibodies
Neoplasms
Cytoplasm
Messenger RNA
Peptides
Brain

Keywords

  • Astrocytoma
  • Glioma
  • Olig1
  • Oligodendroglioma
  • Transcription factor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Immunolocalization of the Oligodendrocyte Transcription Factor 1 (Olig1) in Brain Tumors. / Azzarelli, Biagio; Miravalle, Leticia; Vidal, Ruben.

In: Journal of Neuropathology and Experimental Neurology, Vol. 63, No. 2, 02.2004, p. 170-179.

Research output: Contribution to journalArticle

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