Immunologic comparison of phospholipases A present in Hymenoptera insect venoms

B. C. Nair, Chitra Nair, Scott Denne, John Wypych, C. E. Arbesman, W. B. Elliott

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A comparative study was made of the effect of anti-bee venom phospholipase A2 serum and antir-bald face hornet venom serum on the activity of phospholipase A2 present in yellow hornet, yellow jacket, bald face hornet, and honeybee venoms. Activity of phospholipase A2 present in the venoms was measured titrimetrically using egg yolk dispersion as the substrate. Antiserum against pure bee venom phospholipase A2 activity completely suppressed bee venom phospholipase A2 activity but failed to inhibit phospholipase A2 activity in yellow jacket, yellow hornet, and bald face hornet venoms. Anti-bald face hornet venom serum suppressed the activity of phospholipase A2 present in the four insect venoms with the inhibitory effect decreasing in the order: bald face hornet > yellow hornet > yellow jacket > honeybee. A combination of immunoelectrophoresis and immunodiffusion techniques gave indication of common antigenic sites in a component of yellow hornet venom and a component of bald face hornet venom. The results suggest that IgG antibodies against pure bee venom phospholipase A2 may not give any protection against the reaction(s) due to the phospholipase A2 in yellow hornet, yellow jacket, and bald face hornet venoms, while antibodies against bald face hornet venom phospholipase A2 may give some cross-protection.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume58
Issue number1 PART 1
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Wasps
Hymenoptera
Phospholipases A
Venoms
Insects
Phospholipases A2
Bee Venoms
Serum
Cross Protection
Antivenins
Immunoelectrophoresis
Egg Yolk
Antibodies
Immunodiffusion

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Immunologic comparison of phospholipases A present in Hymenoptera insect venoms. / Nair, B. C.; Nair, Chitra; Denne, Scott; Wypych, John; Arbesman, C. E.; Elliott, W. B.

In: Journal of Allergy and Clinical Immunology, Vol. 58, No. 1 PART 1, 1976, p. 101-109.

Research output: Contribution to journalArticle

Nair, B. C. ; Nair, Chitra ; Denne, Scott ; Wypych, John ; Arbesman, C. E. ; Elliott, W. B. / Immunologic comparison of phospholipases A present in Hymenoptera insect venoms. In: Journal of Allergy and Clinical Immunology. 1976 ; Vol. 58, No. 1 PART 1. pp. 101-109.
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