Immunomagnetic isolation of human developing motor neurons

Vincenzo Silani, Andrea Brioschi, Massimiliano Braga, Andrea Ciammola, Feng Zhou, Christiana Bonifati, Antonia Ratti, Antonio Pizzuti, Mauro Buscaglia, Guglielmo Scarlato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

HUMAN motor neuron (MN) isolation provides a critical tool to study neurophysiological properties and the effects of molecules of clinical relevance on isolated neurons. We developed an immunomagnetic separation technique based on specific MN antigen recognition for nerve growth factor receptor (p(75-NGFR)). We cultured an average of 250 000 cells from the anterior horns of a single cord (four specimens at postconception Weeks 6.0, 7.2, 8.0, and 8.3). At day 7 in vitro (DIV), choline acetyltransferase (CHAT) and/or p(75-NGFR)-expressing cells (MNs) represented 72 ± 2% of the total growing cells. MNs survived for at least 4 weeks in biochemically defined medium. The immunomagnetic separation method has been demonstrated to be effective, reproducible, and quantitative for separation of MNs.

Original languageEnglish
Pages (from-to)1143-1147
Number of pages5
JournalNeuroReport
Volume9
Issue number6
StatePublished - Apr 20 1998

Fingerprint

Immunomagnetic Separation
Motor Neurons
Anterior Horn Cells
Nerve Growth Factor Receptor
Choline O-Acetyltransferase
Neurons
Antigens
In Vitro Techniques

Keywords

  • CHAT
  • Isolation
  • Motor neuron
  • p(75-NGFR)
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Silani, V., Brioschi, A., Braga, M., Ciammola, A., Zhou, F., Bonifati, C., ... Scarlato, G. (1998). Immunomagnetic isolation of human developing motor neurons. NeuroReport, 9(6), 1143-1147.

Immunomagnetic isolation of human developing motor neurons. / Silani, Vincenzo; Brioschi, Andrea; Braga, Massimiliano; Ciammola, Andrea; Zhou, Feng; Bonifati, Christiana; Ratti, Antonia; Pizzuti, Antonio; Buscaglia, Mauro; Scarlato, Guglielmo.

In: NeuroReport, Vol. 9, No. 6, 20.04.1998, p. 1143-1147.

Research output: Contribution to journalArticle

Silani, V, Brioschi, A, Braga, M, Ciammola, A, Zhou, F, Bonifati, C, Ratti, A, Pizzuti, A, Buscaglia, M & Scarlato, G 1998, 'Immunomagnetic isolation of human developing motor neurons', NeuroReport, vol. 9, no. 6, pp. 1143-1147.
Silani V, Brioschi A, Braga M, Ciammola A, Zhou F, Bonifati C et al. Immunomagnetic isolation of human developing motor neurons. NeuroReport. 1998 Apr 20;9(6):1143-1147.
Silani, Vincenzo ; Brioschi, Andrea ; Braga, Massimiliano ; Ciammola, Andrea ; Zhou, Feng ; Bonifati, Christiana ; Ratti, Antonia ; Pizzuti, Antonio ; Buscaglia, Mauro ; Scarlato, Guglielmo. / Immunomagnetic isolation of human developing motor neurons. In: NeuroReport. 1998 ; Vol. 9, No. 6. pp. 1143-1147.
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