Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels: A sensitive new technique

David Mattson, R. P. Roos, B. G W Arnason

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An isoelectric focusing (IEF) technique using direct peroxidase of peroxidase-antiperoxidase (PAP) overlay for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a specific stain. The direct peroxidase system is 4 times and the PAP system 8 times as sensitive as Coomassie blue staining after standard IEF or Coomassie blue staining of immunofixed gels. As little as 0.5 μg (direct peroxidase) or 0.3 μg (PAP) of IgG can be detected. Direct peroxidase staining of IgG in IEF gels can be used to examine unconcentrated CSF, with banding patterns similar to those obtained by Coomassie blue staining after standard IEF of concentrated CSF.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalJournal of Neuroscience Methods
Volume3
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Isoelectric Focusing
Peroxidase
Cerebrospinal Fluid
Immunoglobulin G
Gels
Staining and Labeling
Oligoclonal Bands
Immune Sera
Coloring Agents
Coomassie blue

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels : A sensitive new technique. / Mattson, David; Roos, R. P.; Arnason, B. G W.

In: Journal of Neuroscience Methods, Vol. 3, No. 1, 1980, p. 67-75.

Research output: Contribution to journalArticle

@article{243de0fef68846e89055a7b49227940a,
title = "Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels: A sensitive new technique",
abstract = "An isoelectric focusing (IEF) technique using direct peroxidase of peroxidase-antiperoxidase (PAP) overlay for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a specific stain. The direct peroxidase system is 4 times and the PAP system 8 times as sensitive as Coomassie blue staining after standard IEF or Coomassie blue staining of immunofixed gels. As little as 0.5 μg (direct peroxidase) or 0.3 μg (PAP) of IgG can be detected. Direct peroxidase staining of IgG in IEF gels can be used to examine unconcentrated CSF, with banding patterns similar to those obtained by Coomassie blue staining after standard IEF of concentrated CSF.",
author = "David Mattson and Roos, {R. P.} and Arnason, {B. G W}",
year = "1980",
doi = "10.1016/0165-0270(80)90035-7",
language = "English (US)",
volume = "3",
pages = "67--75",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels

T2 - A sensitive new technique

AU - Mattson, David

AU - Roos, R. P.

AU - Arnason, B. G W

PY - 1980

Y1 - 1980

N2 - An isoelectric focusing (IEF) technique using direct peroxidase of peroxidase-antiperoxidase (PAP) overlay for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a specific stain. The direct peroxidase system is 4 times and the PAP system 8 times as sensitive as Coomassie blue staining after standard IEF or Coomassie blue staining of immunofixed gels. As little as 0.5 μg (direct peroxidase) or 0.3 μg (PAP) of IgG can be detected. Direct peroxidase staining of IgG in IEF gels can be used to examine unconcentrated CSF, with banding patterns similar to those obtained by Coomassie blue staining after standard IEF of concentrated CSF.

AB - An isoelectric focusing (IEF) technique using direct peroxidase of peroxidase-antiperoxidase (PAP) overlay for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a specific stain. The direct peroxidase system is 4 times and the PAP system 8 times as sensitive as Coomassie blue staining after standard IEF or Coomassie blue staining of immunofixed gels. As little as 0.5 μg (direct peroxidase) or 0.3 μg (PAP) of IgG can be detected. Direct peroxidase staining of IgG in IEF gels can be used to examine unconcentrated CSF, with banding patterns similar to those obtained by Coomassie blue staining after standard IEF of concentrated CSF.

UR - http://www.scopus.com/inward/record.url?scp=0019193353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019193353&partnerID=8YFLogxK

U2 - 10.1016/0165-0270(80)90035-7

DO - 10.1016/0165-0270(80)90035-7

M3 - Article

C2 - 6164880

AN - SCOPUS:0019193353

VL - 3

SP - 67

EP - 75

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1

ER -