Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels: A sensitive new technique

David H. Mattson, Raymond P. Roos, Barry G.W. Arnason

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a specific stain. The direct peroxidase system is 4 times and the PAP system 8 times as sensitive as Coomassie blue staining after standard IEF or Coomassie blue staining of immunofixed gels. As little as 0.5 μg (direct peroxidase) or 0.3 μg (PAP) of IgG can be detected. Direct peroxidase staining of IgG in IEF gels can be used to examine unconcentrated CSF, with banding patterns similar to those obtained by Coomassie blue staining after standard IEF of concentrated CSF.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalJournal of Neuroscience Methods
Volume3
Issue number1
DOIs
StatePublished - 1980

Keywords

  • cerebrospinal fluid
  • immunoperoxidase
  • isoelectric focusing technique
  • multiple sclerosis
  • oligoclonal IgG

ASJC Scopus subject areas

  • Neuroscience(all)

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