Immunoprecipitation of 70S, 50S and 30S ribosomes of Escherichia coli

D. K. Lahiri, D. P. Burma

Research output: Contribution to journalArticle

Abstract

Antibodies were raised in rabbits against 70S ribosomes, 50S and 30S ribosomal subunits individually. Purified immunoglobulins from the antiserum against each of the above ribosomal entities were tested for their capabilities of precipitating 70S, 50S and 30S ribosomes. The observations revealed the following: (i) The antiserum (IgG) raised against 70S ribosomes precipitates 70S ribosomes completely, while partial precipitation is seen with the subunits, the extent of precipitation being more with the 50S subunits than with 30S subunits; addition of 50S subunits to the 30S subunits facilitates the precipitation of 30S subunits by the antibody against 70S ribosomes. (ii) Antiserum against 50S subunits has the ability to immunoprecipitate both 50S and 70S ribosomes to an equal extent. (iii) Antiserum against 30S subunits also has the property of precipitating both 30S and 70S ribosomes. The differences in the structural organisation of the two subunits may account for the differences in their immunoprecipitability.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalJournal of Biosciences
Volume2
Issue number1
DOIs
StatePublished - Mar 1 1980
Externally publishedYes

Keywords

  • Ribosomes
  • antibody
  • immunoprecipitability
  • ribosomal subunits

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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